Detailed information NTG1142554

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: DQL77_RS08425
• Genome accession: NZ_LS483484
• Coordinates: 1687969..1688466 (-)
• Length: 165 a.a.
• NCBI ID: WP_001794055.1
• Uniprot ID: A0A7U7EV69
• Organism: Staphylococcus aureus strain NCTC13277
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1682969..1693466

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL77_RS08400 (NCTC13277_01605)	gcvPB	1683131..1684603 (-)	1473	WP_000202178.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
DQL77_RS08405 (NCTC13277_01606)	gcvPA	1684596..1685942 (-)	1347	WP_000019697.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
DQL77_RS08410 (NCTC13277_01607)	gcvT	1685962..1687053 (-)	1092	WP_000093347.1	glycine cleavage system aminomethyltransferase GcvT	-
DQL77_RS08415	-	1687212..1687736 (-)	525	WP_001015125.1	shikimate kinase	-
DQL77_RS08420	-	1687726..1687872 (-)	147	WP_001789879.1	hypothetical protein	-
DQL77_RS08425 (NCTC13277_01609)	comGF	1687969..1688466 (-)	498	WP_001794055.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL77_RS08430 (NCTC13277_01610)	comGE	1688384..1688683 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
DQL77_RS08435 (NCTC13277_01611)	comGD	1688670..1689116 (-)	447	WP_001791207.1	competence type IV pilus minor pilin ComGD	Machinery gene
DQL77_RS08440 (NCTC13277_01612)	comGC	1689094..1689405 (-)	312	WP_000472257.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL77_RS08445 (NCTC13277_01613)	comGB	1689419..1690489 (-)	1071	WP_000776412.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQL77_RS08450 (NCTC13277_01614)	comGA	1690461..1691435 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL77_RS08455 (NCTC13277_01615)	-	1691487..1692110 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
DQL77_RS08460 (NCTC13277_01616)	-	1692107..1692436 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
DQL77_RS08465 (NCTC13277_01617)	-	1692436..1693422 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19224.98 Da
• Isoelectric Point: 10.2192

>NTDB_id=1142554 DQL77_RS08425 WP_001794055.1 1687969..1688466(-) (comGF) [Staphylococcus aureus strain NCTC13277]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1142554 DQL77_RS08425 WP_001794055.1 1687969..1688466(-) (comGF) [Staphylococcus aureus strain NCTC13277]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A7U7EV69

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97