Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   DQM29_RS14235 Genome accession   NZ_LS483470
Coordinates   3109336..3110772 (+) Length   478 a.a.
NCBI ID   WP_111741302.1    Uniprot ID   A0A2X4V4Z8
Organism   Leminorella richardii strain NCTC12151     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3104336..3115772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM29_RS14205 (NCTC12151_02929) mlaF 3104629..3105432 (-) 804 WP_111741297.1 phospholipid ABC transporter ATP-binding protein MlaF -
  DQM29_RS14210 (NCTC12151_02930) kdsD 3105873..3106841 (+) 969 WP_111741298.1 arabinose-5-phosphate isomerase KdsD -
  DQM29_RS14215 (NCTC12151_02931) kdsC 3106895..3107443 (+) 549 WP_111741299.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  DQM29_RS14220 (NCTC12151_02932) lptC 3107440..3108033 (+) 594 WP_111741300.1 LPS export ABC transporter periplasmic protein LptC -
  DQM29_RS14225 (NCTC12151_02933) lptA 3108017..3108535 (+) 519 WP_232054968.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  DQM29_RS14230 (NCTC12151_02934) lptB 3108586..3109311 (+) 726 WP_111742108.1 LPS export ABC transporter ATP-binding protein -
  DQM29_RS14235 (NCTC12151_02935) rpoN/rpoN1 3109336..3110772 (+) 1437 WP_111741302.1 RNA polymerase factor sigma-54 Machinery gene
  DQM29_RS14240 (NCTC12151_02936) ptsN 3110794..3111315 (+) 522 WP_111741303.1 PTS IIA-like nitrogen regulatory protein PtsN -
  DQM29_RS14245 (NCTC12151_02937) rapZ 3111489..3112361 (+) 873 WP_111741304.1 RNase adapter RapZ -
  DQM29_RS14250 (NCTC12151_02938) rnk 3112668..3113075 (+) 408 WP_111741305.1 nucleoside diphosphate kinase regulator -
  DQM29_RS14255 (NCTC12151_02939) pmbA 3113149..3114489 (-) 1341 WP_111741306.1 metalloprotease PmbA -
  DQM29_RS14260 (NCTC12151_02940) yjgA 3114657..3115217 (+) 561 WP_111741307.1 ribosome biogenesis factor YjgA -

Sequence

Protein


Download         Length: 478 a.a.        Molecular weight: 53666.52 Da        Isoelectric Point: 4.4746

>NTDB_id=1142283 DQM29_RS14235 WP_111741302.1 3109336..3110772(+) (rpoN/rpoN1) [Leminorella richardii strain NCTC12151]
MKQGLQLRLSQQLTMTPQLQQAIRLLQLSTLELQQEIQTALESNPLLEQADVHDEIESREAPETEQLDTSEALQQSDIPE
DLPLDASWDEIYTAGTPSGTRNDYGDDELLVFQGETTLTLQDHLMWQVDLTPFTDLDKAIAIAIIDAVDDAGYLTVSLED
IAESLGQEDETVELDELEAVLKRVQRFDPVGVAARDLRECLLVQLSQLDKTTPGVAEAREIVSEHLTLLGNHDFRTLLKL
TRISESLLKEAMQLIQSLDPRPGQSIHSSESEYVIPDVLVRKVHGKWTVELNGESTPRLRINQHYASMTNTHSASDNQYI
RSHLQDAKWLIKSLESRNDTLLRVTECIVERQEAFFESGPEFMKPMVLADIAQAVDMHESTISRVTTQKFLHSPRGIFEL
KYFFSSHVNTESGGEASSTAIRALVKKMISAENPAKPLSDSKIADMLAEQGMVVARRTVAKYRESLSIPPSNQRKQLI

Nucleotide


Download         Length: 1437 bp        

>NTDB_id=1142283 DQM29_RS14235 WP_111741302.1 3109336..3110772(+) (rpoN/rpoN1) [Leminorella richardii strain NCTC12151]
ATGAAGCAAGGTTTACAGCTCAGGCTAAGCCAACAGTTAACCATGACGCCCCAGCTACAGCAGGCCATTCGCCTGTTGCA
GCTGTCGACGCTTGAGCTACAGCAGGAGATTCAAACCGCGCTGGAAAGTAATCCGCTGCTTGAACAGGCAGACGTTCACG
ACGAAATTGAGTCTCGAGAAGCGCCAGAAACAGAGCAGCTTGATACCAGCGAAGCGCTACAGCAGTCGGATATTCCTGAA
GACCTCCCGCTAGACGCATCCTGGGACGAGATCTACACCGCTGGCACCCCGTCGGGTACACGCAACGACTACGGTGACGA
CGAACTCCTCGTGTTTCAGGGTGAAACCACGCTGACGCTGCAGGATCACCTGATGTGGCAAGTAGATCTCACGCCCTTCA
CCGATCTGGATAAAGCGATTGCGATAGCCATTATCGACGCCGTTGACGACGCGGGTTATCTTACTGTTTCACTAGAAGAC
ATCGCCGAAAGTCTCGGCCAAGAAGATGAAACCGTAGAGCTTGATGAGCTCGAAGCCGTGCTTAAGCGCGTTCAGCGCTT
CGACCCCGTCGGCGTTGCTGCGCGGGATCTGCGCGAGTGCCTGCTGGTTCAGCTGTCCCAGCTGGATAAAACCACGCCGG
GCGTTGCCGAAGCGCGGGAAATCGTCTCTGAGCACCTGACTCTGCTGGGCAATCACGACTTTAGAACGCTATTAAAACTG
ACTCGAATCAGCGAGTCTTTGCTCAAAGAGGCCATGCAGCTGATCCAAAGCCTCGATCCGCGCCCCGGCCAGTCGATACA
CAGTAGCGAATCCGAATACGTTATTCCCGACGTTCTGGTGCGAAAAGTGCACGGTAAATGGACGGTAGAGCTTAACGGAG
AAAGCACGCCGCGCCTGCGCATTAATCAGCACTACGCATCGATGACCAATACTCACAGCGCCAGCGACAATCAGTACATT
CGCAGCCACCTCCAGGACGCAAAATGGCTGATAAAAAGCCTGGAAAGCCGTAACGATACGCTCTTGCGCGTCACTGAGTG
TATCGTTGAGCGGCAGGAAGCTTTTTTTGAGTCCGGCCCTGAGTTTATGAAGCCGATGGTGCTGGCGGATATAGCTCAGG
CTGTCGACATGCATGAATCCACTATCTCGCGGGTTACAACACAGAAATTTCTACACAGCCCGCGGGGCATATTTGAACTA
AAATACTTCTTCTCCAGTCATGTGAATACGGAGAGCGGCGGCGAAGCATCCTCTACCGCCATTCGCGCGCTGGTGAAAAA
AATGATCTCGGCCGAAAACCCCGCCAAACCGCTCAGCGACAGTAAAATCGCCGATATGCTGGCGGAACAGGGAATGGTGG
TCGCCAGAAGGACCGTGGCAAAATACCGAGAGTCGTTATCTATTCCTCCTTCAAACCAGCGCAAACAGCTGATTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(318-476)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(117-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X4V4Z8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.214

100

0.498

Multiple sequence alignment