Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DQN30_RS04845 Genome accession   NZ_LS483458
Coordinates   954571..955113 (-) Length   180 a.a.
NCBI ID   WP_111696494.1    Uniprot ID   A0A2X4R4W3
Organism   Haemophilus haemolyticus strain NCTC10839     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 949571..960113
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN30_RS04815 (NCTC10839_00956) fdx 949972..950313 (+) 342 WP_005629440.1 ISC system 2Fe-2S type ferredoxin -
  DQN30_RS04820 (NCTC10839_00957) iscX 950313..950507 (+) 195 WP_111696490.1 Fe-S cluster assembly protein IscX -
  DQN30_RS04825 (NCTC10839_00958) - 950558..951172 (-) 615 WP_111696491.1 YfgM family protein -
  DQN30_RS04830 (NCTC10839_00959) hisS 951190..952461 (-) 1272 WP_111696492.1 histidine--tRNA ligase -
  DQN30_RS04835 (NCTC10839_00960) ispG 952471..953577 (-) 1107 WP_111696493.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DQN30_RS04840 (NCTC10839_00961) - 953589..954500 (-) 912 WP_111697120.1 RodZ family helix-turn-helix domain-containing protein -
  DQN30_RS04845 (NCTC10839_00962) pilF/pilF2 954571..955113 (-) 543 WP_111696494.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DQN30_RS04850 (NCTC10839_00963) - 955214..956365 (-) 1152 WP_172454032.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DQN30_RS04855 (NCTC10839_00964) - 956613..957491 (+) 879 WP_111696496.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DQN30_RS04860 (NCTC10839_00965) - 957694..958575 (+) 882 WP_111696497.1 metal ABC transporter substrate-binding protein -
  DQN30_RS04865 (NCTC10839_00966) - 958575..959495 (+) 921 WP_005634082.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20842.34 Da        Isoelectric Point: 7.8275

>NTDB_id=1142200 DQN30_RS04845 WP_111696494.1 954571..955113(-) (pilF/pilF2) [Haemophilus haemolyticus strain NCTC10839]
MQTISKQLSAVIFPFIFSACVSQSTIQDFNQQSAAKARVELALGYLQQGNPQLAKINLDKALQHDKDYYLVHSALAHYYQ
QQGEIENAHREYETALKLNNQQGDLHNNFGTFLCRQKQFEQAQQQFQLSLNSPNYYHQADTFENMALCAYSAQKMDIYQQ
ALQKLRQTDSKRAEKFNTFK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=1142200 DQN30_RS04845 WP_111696494.1 954571..955113(-) (pilF/pilF2) [Haemophilus haemolyticus strain NCTC10839]
ATGCAAACAATAAGTAAACAACTCAGTGCGGTGATTTTCCCTTTCATTTTTTCTGCTTGCGTTTCCCAATCAACTATTCA
AGATTTCAATCAACAATCAGCAGCTAAAGCTCGTGTAGAACTTGCACTCGGTTACCTTCAACAAGGTAATCCTCAACTAG
CTAAAATCAACTTAGATAAAGCACTTCAACACGATAAAGATTACTATCTCGTGCATTCAGCGCTTGCGCATTATTATCAA
CAACAAGGTGAAATCGAGAACGCACATCGTGAATATGAAACAGCGTTAAAACTCAATAATCAACAGGGGGACTTACATAA
TAATTTTGGCACATTTTTATGTCGTCAAAAACAATTTGAACAAGCTCAGCAGCAATTTCAACTCTCACTGAATTCCCCTA
ATTATTATCATCAAGCAGATACATTTGAAAATATGGCTCTTTGTGCTTATTCCGCTCAAAAAATGGATATTTATCAACAA
GCATTACAAAAATTGCGACAAACTGATAGTAAACGAGCGGAAAAATTTAATACCTTCAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X4R4W3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

82.778

100

0.828
  pilF Glaesserella parasuis strain SC1401

46.703

100

0.472

Multiple sequence alignment