Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQL20_RS02715 Genome accession   NZ_LS483444
Coordinates   494127..495425 (+) Length   432 a.a.
NCBI ID   WP_006596832.1    Uniprot ID   E7SD11
Organism   Streptococcus australis strain NCTC13166     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 489127..500425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL20_RS02690 (NCTC11371_00534) - 489918..490550 (+) 633 WP_040803116.1 response regulator transcription factor -
  DQL20_RS02695 (NCTC11371_00535) - 490595..492001 (+) 1407 WP_006596952.1 Cof-type HAD-IIB family hydrolase -
  DQL20_RS02700 (NCTC11371_00536) - 491998..492366 (+) 369 WP_006596835.1 S1 RNA-binding domain-containing protein -
  DQL20_RS02705 (NCTC11371_00537) cysK 492418..493347 (-) 930 WP_006596834.1 cysteine synthase A -
  DQL20_RS02710 (NCTC11371_00538) - 493439..494071 (-) 633 WP_006596833.1 YigZ family protein -
  DQL20_RS02715 (NCTC11371_00539) comFA/cflA 494127..495425 (+) 1299 WP_006596832.1 DEAD/DEAH box helicase Machinery gene
  DQL20_RS02720 (NCTC11371_00540) - 495488..496093 (+) 606 WP_006596831.1 ComF family protein -
  DQL20_RS02725 (NCTC11371_00541) hpf 496182..496727 (+) 546 WP_006596830.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49497.76 Da        Isoelectric Point: 8.9399

>NTDB_id=1141732 DQL20_RS02715 WP_006596832.1 494127..495425(+) (comFA/cflA) [Streptococcus australis strain NCTC13166]
MKVEDCYGRLFTKEQVDQEVIEQAQLLPSMIEEKGKLFCNRCGSQIDLEHWKLQIGSYYCRACIQLGRIRGDESLYYFPQ
VAFPKEEVLNWQGTLTPFQSRVSEALVQSLGQEDPVMVHAVTGAGKTEMMYHVVAEVIRGGESVCIATPRIDVCIELYHR
MTRDFTCPISLLHGDSEPYFRTPLVIATTHQLLKFYRAFNLLIIDEVDAFPFVDNAVLYQAVSNAVTKNGKLIYLTATST
KELDKKVKKQEIKRVSLPRRFHGNPLVIPRKVWLKDLRKKVEKKQVPGKLLKLIKDQRKNSFPLILFVSEIELGEKILSL
LRRAFKDEIIELVTSKTENRLELVEKFRNQEITILVSTTILERGVTFPKVDVFVIEANHRLFTKSALVQIAGRVGRSMER
PTGELLFLAEGTSKEMTQAIKEIKEMNREAGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1141732 DQL20_RS02715 WP_006596832.1 494127..495425(+) (comFA/cflA) [Streptococcus australis strain NCTC13166]
ATGAAAGTAGAAGATTGTTATGGACGTCTATTTACAAAGGAGCAGGTAGATCAAGAAGTCATAGAACAAGCCCAGTTGCT
CCCGAGTATGATTGAGGAAAAAGGAAAGTTATTCTGCAATCGTTGTGGAAGCCAGATTGACCTAGAACATTGGAAACTGC
AGATTGGCAGTTATTATTGCCGAGCCTGTATCCAATTAGGGAGGATTAGAGGCGATGAATCCTTATACTATTTCCCACAA
GTAGCCTTTCCTAAAGAGGAAGTCTTGAACTGGCAGGGAACCTTAACGCCCTTTCAAAGTCGAGTCTCAGAAGCTCTGGT
CCAATCGTTAGGACAAGAGGATCCTGTTATGGTGCATGCGGTAACAGGAGCTGGAAAAACAGAGATGATGTATCATGTGG
TCGCAGAAGTGATCCGAGGAGGAGAAAGTGTTTGTATTGCTACGCCTAGAATAGATGTCTGTATCGAACTCTACCATCGT
ATGACAAGGGATTTTACCTGCCCTATTTCTCTCCTGCATGGAGATTCAGAACCCTATTTTCGGACTCCCCTTGTCATAGC
AACCACTCACCAACTACTAAAATTCTATCGGGCCTTTAATCTCCTCATTATTGACGAAGTGGATGCCTTTCCATTTGTTG
ATAATGCTGTGCTCTATCAAGCCGTTTCAAATGCCGTAACTAAGAATGGGAAGCTCATCTATTTGACAGCTACCTCTACA
AAAGAGTTAGATAAAAAAGTCAAAAAACAGGAGATTAAACGCGTTAGCCTACCCAGACGCTTTCACGGAAATCCTCTAGT
GATTCCTAGAAAAGTATGGCTAAAAGATCTACGAAAGAAAGTAGAAAAGAAACAAGTGCCTGGGAAGTTACTCAAGCTAA
TAAAAGACCAACGTAAAAACTCCTTTCCCCTGATCCTATTTGTATCAGAAATCGAATTAGGAGAAAAAATCCTCAGTTTA
TTGAGAAGGGCCTTCAAGGATGAAATCATCGAACTGGTGACCTCTAAAACAGAAAATAGGTTGGAACTGGTAGAAAAATT
TCGCAACCAAGAAATCACTATCCTTGTGTCAACGACCATTCTTGAACGGGGCGTCACCTTCCCTAAAGTCGATGTGTTTG
TGATTGAGGCCAACCATCGATTGTTTACCAAGAGCGCTCTAGTTCAAATCGCTGGTCGTGTGGGAAGAAGTATGGAAAGA
CCCACAGGCGAATTGCTTTTCCTAGCAGAAGGAACCAGCAAAGAAATGACACAAGCTATTAAAGAAATAAAAGAAATGAA
TCGGGAGGCAGGGTTTTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

Type III restriction enzyme, res subunit (ResIII)

(95-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E7SD11

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

61.663

100

0.618
  comFA/cflA Streptococcus pneumoniae Rx1

61.201

100

0.613
  comFA/cflA Streptococcus pneumoniae D39

61.201

100

0.613
  comFA/cflA Streptococcus pneumoniae R6

61.201

100

0.613
  comFA/cflA Streptococcus pneumoniae TIGR4

61.201

100

0.613
  comFA/cflA Streptococcus mitis SK321

60.97

100

0.611
  comFA Lactococcus lactis subsp. cremoris KW2

51.759

92.13

0.477
  comFA Latilactobacillus sakei subsp. sakei 23K

36.512

99.537

0.363

Multiple sequence alignment