Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DQN58_RS00510 Genome accession   NZ_LS483443
Coordinates   93634..94185 (-) Length   183 a.a.
NCBI ID   WP_006719284.1    Uniprot ID   E6KZ38
Organism   Aggregatibacter segnis ATCC 33393 strain NCTC 10977     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 88634..99185
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN58_RS00485 (NCTC10977_00094) - 88839..89291 (-) 453 WP_006719277.1 isoprenylcysteine carboxylmethyltransferase family protein -
  DQN58_RS00490 (NCTC10977_00095) - 89346..89960 (-) 615 WP_006719279.1 YfgM family protein -
  DQN58_RS00495 (NCTC10977_00096) hisS 89970..91247 (-) 1278 WP_006719281.1 histidine--tRNA ligase -
  DQN58_RS00500 (NCTC10977_00097) ispG 91273..92376 (-) 1104 WP_032997983.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DQN58_RS00505 (NCTC10977_00098) - 92387..93418 (-) 1032 WP_006719283.1 RodZ family helix-turn-helix domain-containing protein -
  DQN58_RS00510 (NCTC10977_00099) pilF/pilF2 93634..94185 (-) 552 WP_006719284.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DQN58_RS00515 (NCTC10977_00100) - 94210..95364 (-) 1155 WP_006719285.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DQN58_RS00520 (NCTC10977_00101) - 95603..96121 (-) 519 WP_006719287.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 183 a.a.        Molecular weight: 20931.99 Da        Isoelectric Point: 8.8722

>NTDB_id=1141681 DQN58_RS00510 WP_006719284.1 93634..94185(-) (pilF/pilF2) [Aggregatibacter segnis ATCC 33393 strain NCTC 10977]
MVHFSFFTRIIPFIFPFLFSACVSQQVSSDFDKQQAAKARVELALGYLQQGDHNQAKANLDRAISYAPGYYLVHAARAYF
YQQQGDVIQAEQAYLTAIKLDKKQGDVFNNFGTFLCLQGKYSAAYEQFYKALKAPNYYRQGDTYENLALCALSAKDLMVY
QENLLILEKMEPERAKKLALRAK

Nucleotide


Download         Length: 552 bp        

>NTDB_id=1141681 DQN58_RS00510 WP_006719284.1 93634..94185(-) (pilF/pilF2) [Aggregatibacter segnis ATCC 33393 strain NCTC 10977]
ATGGTTCATTTCTCGTTCTTTACTCGAATCATACCGTTTATTTTCCCCTTTCTTTTTTCTGCCTGTGTTTCTCAACAGGT
ATCATCTGATTTTGATAAACAACAGGCGGCAAAAGCCCGTGTTGAATTGGCATTAGGCTATTTGCAGCAAGGGGATCACA
ACCAAGCCAAGGCAAATTTAGACAGGGCGATTTCTTATGCGCCAGGTTATTATTTAGTACATGCGGCGCGCGCTTATTTT
TATCAGCAACAGGGCGATGTGATTCAGGCAGAACAAGCCTATTTGACTGCCATTAAGTTAGATAAAAAACAAGGTGATGT
TTTCAACAATTTCGGTACCTTTCTTTGTTTGCAAGGTAAATATTCTGCCGCCTATGAGCAATTCTATAAAGCATTAAAGG
CGCCTAATTATTATCGGCAGGGTGATACTTATGAAAATCTTGCACTCTGTGCTTTGTCAGCAAAGGATTTAATGGTTTAT
CAGGAAAATCTGTTAATACTTGAAAAAATGGAACCGGAACGGGCAAAGAAATTAGCGTTACGCGCTAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(75-103)

Tetratricopeptide repeat (TPR_8)

(38-68)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB E6KZ38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

55.488

89.617

0.497
  pilF Glaesserella parasuis strain SC1401

42.473

100

0.432

Multiple sequence alignment