Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   T303_RS02955 Genome accession   NZ_CP006819
Coordinates   528049..529368 (+) Length   439 a.a.
NCBI ID   WP_024704193.1    Uniprot ID   -
Organism   Streptococcus thermophilus ASCC 1275     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 523049..534368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  T303_RS02925 (T303_02945) - 523903..524667 (+) 765 WP_002949740.1 ABC transporter ATP-binding protein -
  T303_RS02930 (T303_02950) - 524667..525377 (+) 711 WP_011680767.1 ABC transporter ATP-binding protein -
  T303_RS10030 (T303_02955) - 525513..526173 (+) 661 Protein_523 CBS and ACT domain-containing protein -
  T303_RS02945 (T303_02960) cysK 526340..527266 (-) 927 WP_011680768.1 cysteine synthase A -
  T303_RS02950 (T303_02965) - 527368..527994 (-) 627 WP_011680769.1 YigZ family protein -
  T303_RS02955 (T303_02970) comFA/cflA 528049..529368 (+) 1320 WP_024704193.1 DEAD/DEAH box helicase Machinery gene
  T303_RS02960 (T303_02975) - 529349..530011 (+) 663 WP_011680771.1 ComF family protein -
  T303_RS02965 (T303_02980) raiA 530090..530639 (+) 550 Protein_528 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50601.74 Da        Isoelectric Point: 9.7105

>NTDB_id=114083 T303_RS02955 WP_024704193.1 528049..529368(+) (comFA/cflA) [Streptococcus thermophilus ASCC 1275]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPRFFWQGRLQKSLTSEKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSVT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELTILVTTTILERGVTFPQVDVFVCMANHHLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCRKEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=114083 T303_RS02955 WP_024704193.1 528049..529368(+) (comFA/cflA) [Streptococcus thermophilus ASCC 1275]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTGACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTCTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGAGAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTGTTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGACAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGAAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-206)

Helicase conserved C-terminal domain (Helicase_C)

(290-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

55.452

98.178

0.544
  comFA/cflA Streptococcus pneumoniae Rx1

55.452

98.178

0.544
  comFA/cflA Streptococcus pneumoniae D39

55.452

98.178

0.544
  comFA/cflA Streptococcus pneumoniae R6

55.452

98.178

0.544
  comFA/cflA Streptococcus mitis NCTC 12261

55.116

97.95

0.54
  comFA/cflA Streptococcus mitis SK321

54.186

97.95

0.531
  comFA Lactococcus lactis subsp. cremoris KW2

45.714

95.672

0.437
  comFA Latilactobacillus sakei subsp. sakei 23K

37.011

99.089

0.367

Multiple sequence alignment