Detailed information NTG1140441

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: DQM86_RS01425
• Genome accession: NZ_LS483407
• Coordinates: 261009..262079 (+)
• Length: 356 a.a.
• NCBI ID: WP_002992231.1
• Uniprot ID: A0A5S4TWS5
• Organism: Streptococcus pyogenes strain NCTC13744
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 256009..267079

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM86_RS01410 (NCTC13744_00281)	amiA	256537..258507 (+)	1971	WP_002992224.1	peptide ABC transporter substrate-binding protein	Regulator
DQM86_RS01415 (NCTC13744_00282)	amiC	258572..260074 (+)	1503	WP_002992227.1	ABC transporter permease	Regulator
DQM86_RS01420 (NCTC13744_00283)	amiD	260074..261000 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
DQM86_RS01425 (NCTC13744_00284)	amiE	261009..262079 (+)	1071	WP_002992231.1	ABC transporter ATP-binding protein	Regulator
DQM86_RS01430 (NCTC13744_00285)	amiF	262072..262995 (+)	924	WP_109828742.1	ABC transporter ATP-binding protein	Regulator
DQM86_RS09555	-	263033..263116 (-)	84	Protein_231	IS3 family transposase	-
DQM86_RS09320	-	263143..263394 (-)	252	WP_194088246.1	transposase	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39680.74 Da
• Isoelectric Point: 5.4774

>NTDB_id=1140441 DQM86_RS01425 WP_002992231.1 261009..262079(+) (amiE) [Streptococcus pyogenes strain NCTC13744]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSNAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=1140441 DQM86_RS01425 WP_002992231.1 261009..262079(+) (amiE) [Streptococcus pyogenes strain NCTC13744]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTAATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A5S4TWS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537