Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   DQM65_RS04180 Genome accession   NZ_LS483392
Coordinates   802876..803415 (-) Length   179 a.a.
NCBI ID   WP_111691323.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC11931     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 797876..808415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM65_RS04150 (NCTC11931_00830) fdx 798279..798620 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  DQM65_RS04155 (NCTC11931_00831) iscX 798620..798814 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  DQM65_RS04160 (NCTC11931_00832) - 798865..799479 (-) 615 WP_111691319.1 YfgM family protein -
  DQM65_RS04165 (NCTC11931_00833) hisS 799497..800768 (-) 1272 WP_111691320.1 histidine--tRNA ligase -
  DQM65_RS04170 (NCTC11931_00834) ispG 800778..801884 (-) 1107 WP_111691321.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  DQM65_RS04175 (NCTC11931_00835) - 801893..802804 (-) 912 WP_111691322.1 RodZ family helix-turn-helix domain-containing protein -
  DQM65_RS04180 (NCTC11931_00836) pilF/pilF2 802876..803415 (-) 540 WP_111691323.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  DQM65_RS04185 (NCTC11931_00837) - 803516..804667 (-) 1152 WP_172454809.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  DQM65_RS04190 (NCTC11931_00838) - 804913..805791 (+) 879 WP_075985546.1 D-alanyl-D-alanine carboxypeptidase family protein -
  DQM65_RS04195 (NCTC11931_00839) - 805936..807072 (-) 1137 WP_111691325.1 RNA-guided endonuclease TnpB family protein -
  DQM65_RS04200 (NCTC11931_00840) - 807448..808329 (+) 882 WP_005669100.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20554.24 Da        Isoelectric Point: 8.9421

>NTDB_id=1140043 DQM65_RS04180 WP_111691323.1 802876..803415(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC11931]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAKKMNIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1140043 DQM65_RS04180 WP_111691323.1 802876..803415(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC11931]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCAGCATCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

96.089

100

0.961
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment