Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQM63_RS08825 Genome accession   NZ_LS483385
Coordinates   1764356..1765654 (-) Length   432 a.a.
NCBI ID   WP_002903259.1    Uniprot ID   F2CF63
Organism   Streptococcus sanguinis strain NCTC7863     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1759356..1770654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM63_RS08815 (NCTC7863_01737) hpf 1763070..1763612 (-) 543 WP_002903257.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DQM63_RS08820 (NCTC7863_01738) - 1763694..1764359 (-) 666 WP_002903258.1 ComF family protein -
  DQM63_RS08825 (NCTC7863_01739) comFA/cflA 1764356..1765654 (-) 1299 WP_002903259.1 DEAD/DEAH box helicase Machinery gene
  DQM63_RS08830 (NCTC7863_01740) - 1765710..1766342 (+) 633 WP_002903261.1 YigZ family protein -
  DQM63_RS08835 (NCTC7863_01741) cysK 1766443..1767372 (+) 930 WP_002903262.1 cysteine synthase A -
  DQM63_RS08840 (NCTC7863_01742) - 1767477..1767839 (-) 363 WP_002901252.1 S1 RNA-binding domain-containing protein -
  DQM63_RS08845 (NCTC7863_01743) - 1767839..1769239 (-) 1401 WP_002903263.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  DQM63_RS08850 (NCTC7863_01744) - 1769279..1769911 (-) 633 WP_002903264.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49493.31 Da        Isoelectric Point: 8.6283

>NTDB_id=1139643 DQM63_RS08825 WP_002903259.1 1764356..1765654(-) (comFA/cflA) [Streptococcus sanguinis strain NCTC7863]
MLELQDCLGRIFTESQLESSLRAQAKEIPSIREEKGKLHCGRCNSLIERENQLPVGAYYCRECLILGRVRSDQKLYYFPQ
EDFPKKQVLKWQGQLTEFQGKVSKGLLDALEKRQNTLVHAVTGAGKTEMIYQVIARVIDRGGSVCLASPRIDVCLELYRR
LKEDFSCEIALLHGESEAYFRSPLVIATTHQLLKFYRAFDLLIVDEVDAFPYVDNPILYHAVERSVKEDATTIFLTATST
DELDKRVQKGELKRLSLPRRFHGNPLTIPQKIWLSDFQKHLQKKKIAPKLLKYIQKQRETQFPLLIFAAEIQRGEDVAHA
LQIALPDEKLAFVASTTENRLEIVEQFRRKEITILVTTTILERGVTFPGVDVFVLEANHRLFSRSALVQISGRVGRSMDR
PTGQLLFFHDGTTLAMEKAIREMRDMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1139643 DQM63_RS08825 WP_002903259.1 1764356..1765654(-) (comFA/cflA) [Streptococcus sanguinis strain NCTC7863]
ATGTTAGAGTTGCAAGATTGTTTAGGACGTATATTTACAGAGAGTCAGTTAGAGTCTAGCTTGCGAGCACAAGCAAAAGA
AATTCCCAGTATCCGAGAAGAAAAAGGCAAGCTCCACTGCGGACGCTGCAACAGCTTGATTGAACGAGAAAATCAGCTAC
CTGTTGGAGCTTATTATTGTAGAGAATGCTTAATATTGGGCCGGGTACGGAGTGACCAAAAACTTTACTATTTTCCTCAA
GAGGATTTTCCTAAAAAACAGGTGCTTAAATGGCAGGGGCAACTGACAGAGTTTCAGGGGAAGGTCTCCAAAGGCTTACT
GGATGCGCTAGAGAAAAGACAGAATACGTTAGTGCATGCTGTTACTGGCGCTGGAAAAACAGAAATGATTTACCAAGTGA
TTGCAAGAGTTATTGATAGAGGAGGGTCTGTTTGCTTGGCCAGTCCGCGGATTGATGTTTGTTTAGAACTTTATCGGCGC
CTAAAAGAAGACTTTTCTTGTGAAATAGCCCTTCTGCATGGGGAGTCAGAAGCTTATTTTCGCAGCCCCTTGGTAATTGC
GACCACCCACCAGCTCTTAAAATTTTATCGGGCTTTTGATTTGTTGATTGTGGACGAGGTAGACGCCTTTCCTTATGTGG
ATAATCCCATACTGTATCACGCTGTAGAGCGTTCAGTTAAAGAGGATGCTACAACTATTTTTCTGACAGCCACTTCCACA
GATGAATTGGATAAAAGAGTTCAAAAAGGTGAGTTGAAACGCCTGAGTCTTCCCAGAAGATTTCATGGTAATCCCTTGAC
CATCCCTCAGAAAATTTGGTTATCTGATTTTCAAAAACATCTTCAGAAGAAGAAAATAGCACCTAAACTTTTAAAATATA
TACAAAAACAGAGGGAGACTCAATTTCCTCTCTTAATATTTGCTGCAGAAATTCAAAGAGGAGAGGATGTTGCTCATGCT
TTGCAGATAGCTCTGCCTGATGAAAAGCTGGCTTTTGTCGCCTCTACAACAGAAAATCGTCTAGAGATTGTCGAGCAGTT
TCGTCGCAAGGAGATCACTATTTTGGTGACAACGACTATCTTAGAGCGTGGAGTAACTTTTCCTGGCGTGGATGTTTTTG
TCTTGGAAGCCAATCATCGCCTGTTCAGTCGTAGTGCTCTGGTTCAGATTTCAGGCCGAGTTGGCCGTAGTATGGATAGA
CCGACTGGACAGTTGCTATTTTTTCATGATGGGACTACCCTTGCTATGGAAAAGGCAATTCGAGAAATGAGGGATATGAA
TAAGGAGGCGGGATTATGA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(109-244)

Helicase conserved C-terminal domain (Helicase_C)

(290-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB F2CF63

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

70.96

98.843

0.701
  comFA/cflA Streptococcus pneumoniae D39

70.96

98.843

0.701
  comFA/cflA Streptococcus pneumoniae R6

70.96

98.843

0.701
  comFA/cflA Streptococcus pneumoniae TIGR4

70.726

98.843

0.699
  comFA/cflA Streptococcus mitis NCTC 12261

70.353

98.38

0.692
  comFA/cflA Streptococcus mitis SK321

69.176

98.38

0.681
  comFA Lactococcus lactis subsp. cremoris KW2

55.025

92.13

0.507
  comFA Latilactobacillus sakei subsp. sakei 23K

38.889

100

0.389

Multiple sequence alignment