Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQM60_RS01855 Genome accession   NZ_LS483366
Coordinates   347469..348788 (+) Length   439 a.a.
NCBI ID   WP_111686476.1    Uniprot ID   A0AB37CPR0
Organism   Streptococcus salivarius strain NCTC7366     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 348769..359036 347469..348788 flank -19


Gene organization within MGE regions


Location: 347469..359036
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM60_RS01855 (NCTC7366_00373) comFA/cflA 347469..348788 (+) 1320 WP_111686476.1 DEAD/DEAH box helicase Machinery gene
  DQM60_RS01860 (NCTC7366_00374) - 348769..349431 (+) 663 WP_111686477.1 ComF family protein -
  DQM60_RS01865 (NCTC7366_00375) hpf 349510..350058 (+) 549 WP_002883786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  DQM60_RS01920 (NCTC7366_00386) - 355980..356912 (+) 933 WP_002886641.1 manganese-dependent inorganic pyrophosphatase -
  DQM60_RS01925 (NCTC7366_00387) - 356967..357626 (+) 660 WP_002886642.1 DUF1803 domain-containing protein -
  DQM60_RS01930 (NCTC7366_00388) - 357656..358189 (-) 534 WP_002886644.1 DUF402 domain-containing protein -
  DQM60_RS01935 (NCTC7366_00389) recX 358260..359036 (-) 777 WP_002886645.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50687.79 Da        Isoelectric Point: 9.0241

>NTDB_id=1138762 DQM60_RS01855 WP_111686476.1 347469..348788(+) (comFA/cflA) [Streptococcus salivarius strain NCTC7366]
MVPKEYYGRLFTKEQLPVDYHSEAFTLESMIKVDKQLRCKRCYSQIEEDWQLPEGQYYCRACIVFGRNQEGKELYYFPSK
TSEIEFPVLKWLGELTPYQAEVSEKLLETYQKQKDSLVHAVTGAGKTEMIYKIVAYVLESKGNVAIASPRVDVCRELFLR
MQRAFTCSISLLHAESEPYDGSPLVIATTHQLLKFYQNFDLVIVDEVDAFPFVGNVMLNHAVEQAKKETGRYIYLTATST
ISLEEQVRLGAIEKHHLARRFHGNPLVLPKFCWQGRLQKFLMRGKLPRPLLYQIKKQRKSKFPLLIFFPNIAIGEKFTSI
LQKYLPDEKMAFISSKSEERSATVEQFREKELSILVTTTILERGVTFPQVDVFVCMANHHLYTSSSLIQIGGRVGRSPDR
PTGKLYFFHEGLSKSMLRCREEIKVMNKKGGFNNEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=1138762 DQM60_RS01855 WP_111686476.1 347469..348788(+) (comFA/cflA) [Streptococcus salivarius strain NCTC7366]
ATGGTACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTGCCAGTGGACTATCACTCAGAGGCTTTTACATT
AGAAAGCATGATAAAGGTCGATAAACAACTTAGATGTAAAAGATGTTACAGTCAGATAGAGGAAGATTGGCAATTACCGG
AAGGTCAGTATTATTGCAGAGCATGTATTGTCTTTGGCAGAAACCAAGAAGGAAAAGAACTTTATTATTTTCCTTCAAAA
ACATCTGAAATTGAATTTCCTGTTTTGAAGTGGTTGGGGGAACTGACTCCCTATCAAGCTGAGGTCTCAGAGAAGCTTTT
AGAAACATATCAAAAACAGAAAGACAGCCTCGTACATGCTGTGACCGGTGCTGGTAAGACCGAGATGATTTATAAAATCG
TTGCCTATGTTCTTGAAAGTAAAGGTAATGTAGCTATCGCAAGTCCTCGGGTTGATGTTTGTCGAGAGTTATTTCTACGT
ATGCAGAGGGCTTTTACTTGTAGTATTTCTCTGCTTCATGCTGAGAGTGAACCATATGATGGTAGTCCGCTTGTAATAGC
TACCACCCATCAATTACTAAAGTTTTACCAGAACTTTGACCTAGTTATCGTTGATGAGGTTGATGCATTCCCTTTTGTAG
GAAATGTCATGTTAAATCATGCTGTGGAGCAGGCAAAGAAGGAAACCGGTCGCTATATTTATTTAACAGCGACTTCTACA
ATATCTTTAGAAGAGCAAGTTCGACTTGGAGCTATAGAAAAACATCACCTTGCCAGACGTTTCCATGGAAATCCTTTAGT
ACTTCCTAAATTCTGCTGGCAAGGAAGATTACAAAAATTTCTAATGAGAGGCAAGCTTCCAAGACCCCTTCTTTATCAGA
TTAAAAAGCAACGTAAATCAAAGTTTCCTCTATTAATATTTTTCCCAAATATAGCGATAGGTGAAAAGTTTACTAGCATT
CTGCAAAAATATCTTCCAGATGAAAAAATGGCCTTTATTTCTTCAAAAAGCGAGGAGCGTTCAGCTACCGTAGAGCAGTT
CCGGGAAAAAGAGTTATCCATCTTAGTGACAACAACTATCCTTGAACGTGGTGTAACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATACACTAGTTCGAGTCTTATTCAGATAGGAGGTCGAGTTGGAAGATCCCCAGATAGA
CCGACAGGAAAGCTTTATTTCTTTCATGAAGGACTATCCAAATCTATGCTTCGTTGTCGAGAAGAAATAAAAGTTATGAA
TAAAAAAGGAGGATTTAACAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-207)

Helicase conserved C-terminal domain (Helicase_C)

(290-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

55.684

98.178

0.547
  comFA/cflA Streptococcus pneumoniae D39

55.684

98.178

0.547
  comFA/cflA Streptococcus pneumoniae R6

55.684

98.178

0.547
  comFA/cflA Streptococcus pneumoniae TIGR4

55.684

98.178

0.547
  comFA/cflA Streptococcus mitis NCTC 12261

54.839

98.861

0.542
  comFA/cflA Streptococcus mitis SK321

54.292

98.178

0.533
  comFA Lactococcus lactis subsp. cremoris KW2

45.368

95.9

0.435
  comFA Latilactobacillus sakei subsp. sakei 23K

38.303

99.317

0.38

Multiple sequence alignment