Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQM50_RS03000 Genome accession   NZ_LS483347
Coordinates   565590..566915 (+) Length   441 a.a.
NCBI ID   WP_076634161.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8324     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 560590..571915
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM50_RS02975 (NCTC8324_00598) - 561360..562001 (+) 642 WP_002983671.1 response regulator transcription factor -
  DQM50_RS02980 (NCTC8324_00599) - 562038..563438 (+) 1401 WP_085613761.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  DQM50_RS02985 (NCTC8324_00600) - 563438..563815 (+) 378 WP_023610575.1 S1 RNA-binding domain-containing protein -
  DQM50_RS02990 (NCTC8324_00601) cysK 563833..564774 (-) 942 WP_085613760.1 cysteine synthase A -
  DQM50_RS02995 (NCTC8324_00602) - 564902..565534 (-) 633 WP_032465870.1 YigZ family protein -
  DQM50_RS03000 (NCTC8324_00603) comFA/cflA 565590..566915 (+) 1326 WP_076634161.1 DEAD/DEAH box helicase Machinery gene
  DQM50_RS03005 (NCTC8324_00604) - 566887..567552 (+) 666 WP_111688422.1 ComF family protein -
  DQM50_RS03010 (NCTC8324_00605) hpf 567632..568180 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49774.31 Da        Isoelectric Point: 10.0009

>NTDB_id=1137761 DQM50_RS03000 WP_076634161.1 565590..566915(+) (comFA/cflA) [Streptococcus pyogenes strain NCTC8324]
MESIENSYGRLLLESQLPDSAKQLAQPLKSVVILRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWGGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKDLLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1137761 DQM50_RS03000 WP_076634161.1 565590..566915(+) (comFA/cflA) [Streptococcus pyogenes strain NCTC8324]
ATGGAGTCTATTGAAAATAGTTATGGACGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAAGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATTGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGGGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCAAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGACTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae D39

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae R6

53.938

95.011

0.512
  comFA/cflA Streptococcus pneumoniae TIGR4

53.938

95.011

0.512
  comFA/cflA Streptococcus mitis NCTC 12261

52.607

95.692

0.503
  comFA/cflA Streptococcus mitis SK321

51.896

95.692

0.497
  comFA Lactococcus lactis subsp. cremoris KW2

47.475

89.796

0.426
  comFA Latilactobacillus sakei subsp. sakei 23K

39.31

98.639

0.388

Multiple sequence alignment