Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQL34_RS02230 Genome accession   NZ_LS483339
Coordinates   412507..413826 (+) Length   439 a.a.
NCBI ID   WP_084828509.1    Uniprot ID   A0A0E2Q4Z1
Organism   Streptococcus thermophilus strain NCTC12958     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 407507..418826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL34_RS02205 (NCTC12958_00464) - 408360..409124 (+) 765 WP_111679257.1 ABC transporter ATP-binding protein -
  DQL34_RS02210 (NCTC12958_00465) - 409124..409834 (+) 711 WP_014607989.1 ABC transporter ATP-binding protein -
  DQL34_RS02215 - 409970..410632 (+) 663 Protein_390 CBS and ACT domain-containing protein -
  DQL34_RS02220 (NCTC12958_00468) cysK 410798..411724 (-) 927 WP_014621180.1 cysteine synthase A -
  DQL34_RS02225 (NCTC12958_00469) - 411826..412452 (-) 627 WP_011680769.1 YigZ family protein -
  DQL34_RS02230 (NCTC12958_00470) comFA/cflA 412507..413826 (+) 1320 WP_084828509.1 DEAD/DEAH box helicase Machinery gene
  DQL34_RS02235 (NCTC12958_00471) - 413807..414469 (+) 663 WP_100262556.1 ComF family protein -
  DQL34_RS02240 (NCTC12958_00472) hpf 414548..415096 (+) 549 WP_084828511.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 439 a.a.        Molecular weight: 50600.69 Da        Isoelectric Point: 9.7105

>NTDB_id=1137350 DQL34_RS02230 WP_084828509.1 412507..413826(+) (comFA/cflA) [Streptococcus thermophilus strain NCTC12958]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEIIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPRFFWQGRLQKSLTNEKLPRSLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFPQVDVFVCMANHHLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCRKEINAMNKKGGFENEVSTM

Nucleotide


Download         Length: 1320 bp        

>NTDB_id=1137350 DQL34_RS02230 WP_084828509.1 412507..413826(+) (comFA/cflA) [Streptococcus thermophilus strain NCTC12958]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGACTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATAATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGATGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTCGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAACGAGAAGCTTCCAAGGTCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTTCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTCCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGAAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

Type III restriction enzyme, res subunit (ResIII)

(94-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E2Q4Z1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae TIGR4

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae Rx1

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae D39

54.988

98.178

0.54
  comFA/cflA Streptococcus pneumoniae R6

54.988

98.178

0.54
  comFA/cflA Streptococcus mitis NCTC 12261

54.651

97.95

0.535
  comFA/cflA Streptococcus mitis SK321

53.721

97.95

0.526
  comFA Lactococcus lactis subsp. cremoris KW2

45.714

95.672

0.437
  comFA Latilactobacillus sakei subsp. sakei 23K

36.782

99.089

0.364

Multiple sequence alignment