Detailed information NTG1137068

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: DQL28_RS01415
• Genome accession: NZ_LS483334
• Coordinates: 257224..258294 (+)
• Length: 356 a.a.
• NCBI ID: WP_010921893.1
• Uniprot ID: A0A4U9C068
• Organism: Streptococcus pyogenes strain NCTC12050
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 252224..263294

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL28_RS01400 (NCTC12050_00274)	amiA	252752..254722 (+)	1971	WP_111676126.1	peptide ABC transporter substrate-binding protein	Regulator
DQL28_RS01405 (NCTC12050_00275)	amiC	254787..256289 (+)	1503	WP_002991105.1	ABC transporter permease	Regulator
DQL28_RS01410 (NCTC12050_00276)	amiD	256289..257215 (+)	927	WP_111676128.1	oligopeptide ABC transporter permease OppC	Regulator
DQL28_RS01415 (NCTC12050_00277)	amiE	257224..258294 (+)	1071	WP_010921893.1	ABC transporter ATP-binding protein	Regulator
DQL28_RS01420 (NCTC12050_00278)	amiF	258287..259210 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
DQL28_RS09715	-	259248..259337 (-)	90	WP_109821088.1	IS3 family transposase	-
DQL28_RS09425	-	259358..259603 (-)	246	WP_030126956.1	hypothetical protein	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39703.77 Da
• Isoelectric Point: 5.6707

>NTDB_id=1137068 DQL28_RS01415 WP_010921893.1 257224..258294(+) (amiE) [Streptococcus pyogenes strain NCTC12050]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=1137068 DQL28_RS01415 WP_010921893.1 257224..258294(+) (amiE) [Streptococcus pyogenes strain NCTC12050]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGAGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTCATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A4U9C068

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537