Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DQL79_RS08140 Genome accession   NZ_LS483324
Coordinates   1621523..1622020 (-) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain NCTC10344     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1616523..1627020
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL79_RS08115 (NCTC10344_01529) gcvPB 1616685..1618157 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  DQL79_RS08120 (NCTC10344_01530) gcvPA 1618150..1619496 (-) 1347 WP_000019691.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DQL79_RS08125 (NCTC10344_01531) gcvT 1619516..1620607 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  DQL79_RS08130 - 1620766..1621290 (-) 525 WP_001015121.1 shikimate kinase -
  DQL79_RS08135 - 1621280..1621426 (-) 147 WP_001789879.1 hypothetical protein -
  DQL79_RS08140 (NCTC10344_01533) comGF 1621523..1622020 (-) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL79_RS08145 (NCTC10344_01534) comGE 1621938..1622237 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DQL79_RS08150 (NCTC10344_01535) comGD 1622224..1622670 (-) 447 WP_072433556.1 competence type IV pilus minor pilin ComGD Machinery gene
  DQL79_RS08155 (NCTC10344_01536) comGC 1622648..1622959 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL79_RS08160 (NCTC10344_01537) comGB 1622973..1624043 (-) 1071 WP_000776421.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL79_RS08165 (NCTC10344_01538) comGA 1624015..1624989 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL79_RS08170 (NCTC10344_01539) - 1625041..1625664 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DQL79_RS08175 (NCTC10344_01540) - 1625661..1625990 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DQL79_RS08180 (NCTC10344_01541) - 1625990..1626976 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1136522 DQL79_RS08140 WP_029694050.1 1621523..1622020(-) (comGF) [Staphylococcus aureus strain NCTC10344]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1136522 DQL79_RS08140 WP_029694050.1 1621523..1622020(-) (comGF) [Staphylococcus aureus strain NCTC10344]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97

Multiple sequence alignment