Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   O987_RS25960 Genome accession   NZ_CP006704
Coordinates   5722310..5723911 (-) Length   533 a.a.
NCBI ID   WP_043375674.1    Uniprot ID   A0A076PRK7
Organism   Comamonas testosteroni TK102     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5717310..5728911
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O987_RS25940 (O987_26920) - 5717537..5718769 (+) 1233 WP_043375669.1 DUF711 family protein -
  O987_RS25945 (O987_26925) - 5718904..5719830 (+) 927 WP_080731616.1 aspartyl/asparaginyl beta-hydroxylase domain-containing protein -
  O987_RS25950 (O987_26930) - 5720339..5720902 (+) 564 WP_144244977.1 hypothetical protein -
  O987_RS29620 (O987_26935) - 5720946..5722229 (+) 1284 WP_235214222.1 hypothetical protein -
  O987_RS25960 (O987_26940) rpoN/rpoN1 5722310..5723911 (-) 1602 WP_043375674.1 RNA polymerase factor sigma-54 Machinery gene
  O987_RS25965 (O987_26945) lptB 5724057..5724839 (-) 783 WP_003060565.1 LPS export ABC transporter ATP-binding protein -
  O987_RS25970 (O987_26950) lptA 5724836..5725492 (-) 657 WP_029158548.1 lipopolysaccharide transport periplasmic protein LptA -
  O987_RS25975 (O987_26955) - 5725623..5726273 (-) 651 WP_003060558.1 thiol:disulfide interchange protein DsbA/DsbL -
  O987_RS25980 (O987_26960) - 5726429..5727133 (-) 705 WP_003060555.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 533 a.a.        Molecular weight: 59029.31 Da        Isoelectric Point: 4.6632

>NTDB_id=113604 O987_RS25960 WP_043375674.1 5722310..5723911(-) (rpoN/rpoN1) [Comamonas testosteroni TK102]
MKPGLSLRVSQHLSLTPQLQQSIQLLQLSTLELAQEVEQMLVDNPFLERAGDEAETAGDAPLDVASSASEYSTSDSIHAE
KEASQAQLATETVAEAPSPETPETLNWEADGMDGQDGEWGGESSSEWDTPSASSSTRNRDGDDETDAIDLAREHQSLTDF
LHRQALSLHLGAADTAALRFLIESLSDDGYLEDSLQELAQSLAGDDLEEREELEQHFAIALKLLQSLEPVGVGARDLAEC
LQLQLRDLIRTAEDEGTTEAHMNRLLLAQAICAQPMDLLARRDLRKLTSLCGAREEQLREAMALIARLEPRPGRRFVDVE
RHIVVPDVIVRPVRAGSGRADFSVQLNPEVMPRLRVHDIYAGVLRRHKNSDGHAALQQQLQEARWFIKNIQQRFDTILRV
SEAIVERQKNFFVHGELAMRPLVLRDIADTLGLHESTISRVTTAKYMATPQGTFELKYFFGSGLGTETGGSASSTAVRAL
IKQFVDAEDARKPLSDAKLADMLKEQGIECARRTVAKYREQLKIPTTTLRKAL

Nucleotide


Download         Length: 1602 bp        

>NTDB_id=113604 O987_RS25960 WP_043375674.1 5722310..5723911(-) (rpoN/rpoN1) [Comamonas testosteroni TK102]
ATGAAGCCAGGTCTCTCGCTACGCGTTTCGCAGCATTTATCACTGACGCCGCAGCTGCAGCAATCCATTCAGCTGCTGCA
GCTCTCCACTCTGGAGCTGGCGCAGGAAGTCGAGCAGATGCTGGTCGACAACCCTTTTCTGGAGCGTGCGGGCGATGAGG
CCGAAACTGCGGGAGATGCTCCTTTAGATGTAGCTAGTAGCGCCAGTGAATACAGTACTTCAGATAGTATTCATGCTGAA
AAAGAAGCATCGCAAGCGCAATTAGCTACTGAAACAGTAGCGGAAGCACCGAGCCCCGAAACCCCGGAAACCCTGAACTG
GGAGGCCGACGGCATGGACGGCCAGGACGGCGAATGGGGCGGCGAATCCAGCAGCGAGTGGGATACGCCCTCGGCCAGCA
GCTCCACGCGCAACCGCGATGGCGATGACGAAACCGATGCCATCGATCTGGCGCGCGAGCACCAGAGCCTGACCGACTTT
CTCCATCGTCAGGCGCTGAGCCTGCATCTGGGCGCGGCAGACACGGCGGCGCTGCGCTTTCTCATCGAGTCGCTCAGCGA
CGACGGCTATCTCGAGGATTCCCTGCAGGAGCTGGCGCAGAGCCTGGCCGGCGACGATCTGGAAGAGCGCGAGGAGCTGG
AGCAGCACTTTGCCATTGCGCTGAAGCTGCTGCAAAGCCTGGAGCCGGTGGGCGTGGGCGCGCGCGATCTGGCCGAGTGC
CTGCAGCTGCAGCTGCGCGACCTGATCCGCACGGCCGAGGACGAGGGCACGACCGAGGCGCATATGAACCGGCTGCTGCT
GGCGCAGGCCATCTGCGCGCAGCCCATGGACTTGCTGGCGCGGCGCGATCTGCGCAAGCTCACCAGCCTGTGCGGCGCGC
GCGAGGAACAGCTGCGCGAGGCCATGGCCCTGATCGCCAGGCTGGAGCCGCGTCCGGGACGCCGCTTTGTCGACGTGGAG
CGCCATATCGTGGTGCCCGATGTCATCGTGCGCCCGGTGCGGGCCGGCTCGGGCAGGGCAGACTTTTCCGTGCAGCTCAA
CCCCGAGGTCATGCCGCGTCTGCGCGTGCACGACATCTATGCCGGAGTGCTGCGTCGCCACAAGAACAGCGACGGTCATG
CGGCGCTGCAGCAGCAGCTGCAGGAGGCGCGCTGGTTCATCAAGAACATCCAGCAGCGCTTCGACACCATCTTGCGTGTT
TCCGAAGCCATCGTGGAGCGGCAGAAGAACTTCTTTGTGCATGGCGAGCTGGCCATGCGCCCGCTCGTACTGCGCGATAT
CGCCGACACGCTGGGCCTGCACGAGTCCACCATCAGCCGCGTGACCACGGCCAAGTACATGGCCACGCCCCAAGGTACTT
TCGAGCTCAAGTATTTCTTCGGTTCGGGCCTGGGCACGGAAACCGGCGGCAGTGCCTCCAGCACGGCCGTACGTGCGCTG
ATCAAACAGTTTGTCGATGCCGAAGATGCCAGAAAGCCGCTGTCGGACGCCAAGCTGGCCGACATGCTCAAGGAGCAGGG
CATTGAGTGTGCGCGCCGCACTGTCGCCAAATACCGCGAGCAGCTCAAGATACCGACGACCACGCTGCGCAAGGCGCTGT
AG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(375-531)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(150-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A076PRK7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.54

100

0.505

Multiple sequence alignment