Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   DQL96_RS08285 Genome accession   NZ_LS483309
Coordinates   1648828..1649325 (-) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain NCTC9944     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1643828..1654325
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL96_RS08260 (NCTC9944_01557) gcvPB 1643990..1645462 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  DQL96_RS08265 (NCTC9944_01558) gcvPA 1645455..1646801 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  DQL96_RS08270 (NCTC9944_01559) gcvT 1646821..1647912 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  DQL96_RS08275 - 1648071..1648595 (-) 525 WP_001015117.1 shikimate kinase -
  DQL96_RS08280 - 1648585..1648731 (-) 147 WP_001789879.1 hypothetical protein -
  DQL96_RS08285 (NCTC9944_01561) comGF 1648828..1649325 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL96_RS08290 (NCTC9944_01562) comGE 1649243..1649542 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  DQL96_RS08295 (NCTC9944_01563) comGD 1649529..1649975 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  DQL96_RS08300 (NCTC9944_01564) comGC 1649953..1650264 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL96_RS08305 (NCTC9944_01565) comGB 1650278..1651348 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL96_RS08310 (NCTC9944_01566) comGA 1651320..1652294 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL96_RS08315 (NCTC9944_01567) - 1652346..1652969 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  DQL96_RS08320 (NCTC9944_01568) - 1652966..1653295 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  DQL96_RS08325 (NCTC9944_01569) - 1653295..1654281 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=1135864 DQL96_RS08285 WP_001803317.1 1648828..1649325(-) (comGF) [Staphylococcus aureus strain NCTC9944]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1135864 DQL96_RS08285 WP_001803317.1 1648828..1649325(-) (comGF) [Staphylococcus aureus strain NCTC9944]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994
  comGF Staphylococcus aureus N315

95.758

100

0.958

Multiple sequence alignment