Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   DQM39_RS03245 Genome accession   NZ_LS483298
Coordinates   594367..595692 (+) Length   441 a.a.
NCBI ID   WP_002983681.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8225     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589367..600692
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM39_RS03220 (NCTC8225_00648) - 590137..590778 (+) 642 WP_002983671.1 response regulator transcription factor -
  DQM39_RS03225 (NCTC8225_00649) - 590815..592215 (+) 1401 WP_002983672.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  DQM39_RS03230 (NCTC8225_00650) - 592215..592592 (+) 378 WP_002983674.1 S1 RNA-binding domain-containing protein -
  DQM39_RS03235 (NCTC8225_00651) cysK 592610..593551 (-) 942 WP_002983675.1 cysteine synthase A -
  DQM39_RS03240 (NCTC8225_00652) - 593679..594311 (-) 633 WP_002983676.1 YigZ family protein -
  DQM39_RS03245 (NCTC8225_00653) comFA/cflA 594367..595692 (+) 1326 WP_002983681.1 DEAD/DEAH box helicase Machinery gene
  DQM39_RS03250 (NCTC8225_00654) - 595664..596329 (+) 666 WP_002983685.1 ComF family protein -
  DQM39_RS03255 (NCTC8225_00655) hpf 596409..596957 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49792.28 Da        Isoelectric Point: 10.0009

>NTDB_id=1135467 DQM39_RS03245 WP_002983681.1 594367..595692(+) (comFA/cflA) [Streptococcus pyogenes strain NCTC8225]
MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESLPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLAKKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1135467 DQM39_RS03245 WP_002983681.1 594367..595692(+) (comFA/cflA) [Streptococcus pyogenes strain NCTC8225]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAGGCAACTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCATATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAGTTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTCTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCAGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGAGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAAAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(98-244)

Helicase conserved C-terminal domain (Helicase_C)

(289-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae D39

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae R6

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae TIGR4

54.415

95.011

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

53.081

95.692

0.508
  comFA/cflA Streptococcus mitis SK321

52.133

95.692

0.499
  comFA Lactococcus lactis subsp. cremoris KW2

47.368

90.476

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365

Multiple sequence alignment