Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NMK36_RS25255 Genome accession   NZ_LR882967
Coordinates   5834937..5836940 (-) Length   667 a.a.
NCBI ID   WP_072717945.1    Uniprot ID   A0A9W4CXZ9
Organism   Planktothrix pseudagardhii strain No.713     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5829937..5841940
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMK36_RS25230 (NO713_05058) hisA 5830139..5830912 (-) 774 WP_254174909.1 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase -
  NMK36_RS25235 (NO713_05059) - 5831009..5831398 (-) 390 WP_254174910.1 hypothetical protein -
  NMK36_RS25240 (NO713_05060) - 5831541..5832155 (-) 615 WP_254174911.1 hypothetical protein -
  NMK36_RS25245 (NO713_05061) - 5832321..5833454 (-) 1134 WP_254174912.1 type II secretion system F family protein -
  NMK36_RS25250 (NO713_05062) - 5833557..5834660 (-) 1104 WP_072717946.1 type IV pilus twitching motility protein PilT -
  NMK36_RS25255 (NO713_05063) pilB/pilB1 5834937..5836940 (-) 2004 WP_072717945.1 GspE/PulE family protein Machinery gene
  NMK36_RS25260 (NO713_05064) grpE 5837986..5838732 (+) 747 WP_072717944.1 nucleotide exchange factor GrpE -
  NMK36_RS25265 (NO713_05065) dnaK 5838884..5840938 (+) 2055 WP_254174913.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 667 a.a.        Molecular weight: 74886.78 Da        Isoelectric Point: 5.5687

>NTDB_id=1132971 NMK36_RS25255 WP_072717945.1 5834937..5836940(-) (pilB/pilB1) [Planktothrix pseudagardhii strain No.713]
MTNTSQRRALVVQSTTPFGNKLIQSGYINPEQLQQALKTSRSGQPLTEVLESMTGRPLPPDLARQYKKQQLFELRIIHGV
EAYDPEIEPMSNEELEQMLHTLDTSLETCKRHRFIPLRKPESDPTSVLIAMVDPDNLNALEELRNQILRPKQLKLQRRVI
TQEDYDQIISTYSNEDVKRKAAEAAKREQEKLEFTAGDFETTDLEEVGDESGGMDLSEEIEQAETAPVIKAVNVILAKAL
SEGVSDIHVEPQEHEMRIRMRKDGVLQEYYKFPKKVIPAITSRFKILAEMDIAERRQAQDGRIRRKFRGRTVDFRVNSLP
SRYGEKIVLRILDSSSTQLGLDKLITDQETLALVRETASRPFGLILVTGPTGSGKSTSLYSVLAEKNDPGINISTAEDPI
EYALPGITQCQVIREKNLTFANILRAFLRQDPDVLLVGETRDLETAKTAIEAALTGHLVLTTLHTNDAAGAIARLDEMGV
EPFMVSAALLGILAQRLMRRVCGECRLPYTPAPEELGRYGLSASQEVNITFYKANTIAVEERKHLADMGQLCKKCSGLGY
KGRVGVYEFLKVTERMQTLITKGAPTEQIKEAAVEEGMKTLLAYSLQLVREGYTTFEEVERVTFTDSGLEAELKAKRKQG
LTCKSCVAELKPEWLDCPYCMTPRFIS

Nucleotide


Download         Length: 2004 bp        

>NTDB_id=1132971 NMK36_RS25255 WP_072717945.1 5834937..5836940(-) (pilB/pilB1) [Planktothrix pseudagardhii strain No.713]
ATGACGAACACATCCCAAAGACGCGCCCTAGTTGTTCAAAGTACCACTCCCTTTGGCAACAAATTAATTCAATCAGGCTA
TATTAATCCAGAGCAACTTCAGCAAGCCCTCAAAACCAGTCGTTCAGGTCAACCCCTGACAGAAGTTTTGGAATCGATGA
CGGGTCGCCCCCTTCCTCCCGACTTGGCACGTCAGTATAAAAAACAACAGCTATTTGAACTGAGAATCATTCACGGGGTC
GAAGCGTATGATCCTGAAATTGAACCGATGTCGAATGAAGAACTCGAACAAATGCTGCATACGCTTGATACCAGTTTAGA
AACCTGTAAACGCCATCGCTTCATCCCTTTACGCAAACCTGAAAGTGATCCGACTTCGGTGTTGATTGCCATGGTTGACC
CCGACAACCTGAATGCGTTGGAGGAGTTAAGAAATCAAATTTTGCGACCCAAGCAGTTGAAACTCCAACGGCGGGTGATT
ACTCAGGAAGATTATGATCAAATTATTTCCACCTATAGTAACGAAGACGTTAAACGCAAAGCCGCCGAAGCCGCTAAACG
TGAACAAGAGAAATTAGAGTTTACCGCCGGAGATTTTGAAACCACCGACCTCGAAGAAGTAGGCGATGAATCTGGTGGGA
TGGATTTGTCGGAGGAAATCGAACAAGCGGAAACCGCCCCGGTTATTAAGGCTGTCAACGTGATTTTAGCCAAAGCCCTC
TCGGAGGGGGTATCTGATATTCACGTTGAACCCCAGGAACATGAAATGCGAATTCGGATGCGGAAAGATGGGGTGTTACA
GGAATATTATAAATTTCCCAAAAAAGTAATTCCGGCGATTACTTCCCGGTTTAAAATTCTCGCAGAAATGGATATCGCCG
AACGTCGTCAAGCCCAAGATGGTCGGATTCGCCGTAAATTCCGAGGACGGACGGTAGACTTCCGGGTGAATAGTCTACCG
AGTCGCTACGGGGAAAAAATTGTATTACGGATTCTCGATAGTTCTTCAACCCAATTAGGGTTAGATAAATTAATTACGGA
TCAAGAAACCTTAGCCTTAGTTCGGGAAACCGCATCTCGTCCTTTTGGTTTGATATTAGTTACAGGGCCAACGGGGTCAG
GAAAATCCACCTCTCTGTATTCGGTTTTAGCAGAAAAAAATGATCCGGGGATTAATATTAGTACCGCAGAAGATCCGATT
GAATATGCCTTACCTGGGATTACCCAATGTCAGGTAATTCGGGAGAAAAATTTAACCTTTGCTAATATTTTACGGGCATT
TTTACGACAAGACCCGGATGTTTTATTAGTGGGGGAAACACGGGATTTAGAAACAGCAAAAACCGCTATTGAAGCAGCAT
TAACCGGACACTTAGTGTTAACCACATTACACACCAACGACGCAGCAGGTGCCATTGCTCGTTTGGATGAAATGGGGGTA
GAACCGTTCATGGTGTCGGCTGCTTTGTTAGGAATTCTCGCCCAACGGCTGATGCGGCGCGTTTGTGGGGAGTGTCGGCT
TCCTTATACCCCGGCTCCTGAAGAATTAGGTCGTTATGGGTTATCGGCTTCTCAAGAAGTCAATATTACTTTTTATAAAG
CGAATACCATTGCGGTTGAAGAACGGAAGCATTTAGCAGATATGGGCCAATTGTGTAAAAAATGTAGTGGACTCGGCTAC
AAAGGTCGGGTAGGGGTCTATGAATTCCTGAAGGTGACCGAACGAATGCAAACCCTGATTACCAAAGGTGCTCCCACTGA
ACAAATTAAGGAAGCAGCCGTTGAAGAAGGGATGAAAACTTTATTAGCCTATAGTTTGCAGTTAGTCCGTGAAGGGTACA
CCACCTTTGAGGAAGTGGAACGGGTAACGTTTACAGACTCCGGTTTAGAAGCCGAACTCAAAGCCAAACGCAAACAAGGC
TTAACCTGTAAGAGTTGCGTAGCGGAATTAAAACCGGAATGGTTAGACTGTCCCTATTGTATGACACCCCGCTTTATCAG
CTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(22-173)

Type II/IV secretion system protein (T2SSE)

(235-500)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

62.576

98.951

0.619

Multiple sequence alignment