Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   BKMNNEJI_RS02170 Genome accession   NZ_LR822061
Coordinates   403112..403798 (+) Length   228 a.a.
NCBI ID   WP_001802022.1    Uniprot ID   A0A7U7EYP5
Organism   Staphylococcus aureus isolate HU-14     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 398112..408798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BKMNNEJI_RS02135 (LJDFIFNA_00398) yqeH 398639..399739 (+) 1101 WP_001280137.1 ribosome biogenesis GTPase YqeH -
  BKMNNEJI_RS02140 (LJDFIFNA_00399) aroE 399753..400559 (+) 807 WP_000666757.1 shikimate dehydrogenase -
  BKMNNEJI_RS02145 (LJDFIFNA_00400) yhbY 400563..400853 (+) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  BKMNNEJI_RS02150 (LJDFIFNA_00401) nadD 400856..401425 (+) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  BKMNNEJI_RS02155 (LJDFIFNA_00402) yqeK 401415..401999 (+) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  BKMNNEJI_RS02160 (LJDFIFNA_00403) rsfS 402000..402353 (+) 354 WP_001088020.1 ribosome silencing factor -
  BKMNNEJI_RS02165 (LJDFIFNA_00404) - 402356..403072 (+) 717 WP_000084830.1 class I SAM-dependent DNA methyltransferase -
  BKMNNEJI_RS02170 (LJDFIFNA_00405) comEA 403112..403798 (+) 687 WP_001802022.1 ComEA family DNA-binding protein Machinery gene
  BKMNNEJI_RS02175 (LJDFIFNA_00406) - 403890..404351 (+) 462 WP_000439693.1 ComE operon protein 2 -
  BKMNNEJI_RS02180 (LJDFIFNA_00407) comEC 404356..406557 (+) 2202 WP_001557362.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  BKMNNEJI_RS02185 (LJDFIFNA_00408) holA 406614..407588 (+) 975 WP_001282563.1 DNA polymerase III subunit delta -
  BKMNNEJI_RS02190 (LJDFIFNA_00409) rpsT 407633..407884 (-) 252 WP_001274017.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25900.65 Da        Isoelectric Point: 9.6751

>NTDB_id=1132266 BKMNNEJI_RS02170 WP_001802022.1 403112..403798(+) (comEA) [Staphylococcus aureus isolate HU-14]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENNSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLDDADVSRINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1132266 BKMNNEJI_RS02170 WP_001802022.1 403112..403798(+) (comEA) [Staphylococcus aureus isolate HU-14]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAATAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGATGATGCAGATGTAAGTCGAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7EYP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus N315

100

100

1
  comEA Staphylococcus aureus MW2

97.368

100

0.974

Multiple sequence alignment