Detailed information NTG1131420

Bioinformatically predicted

Overview

• Name: comFA/cflA
• Type: Machinery gene
• Locus tag: H1W89_RS07670
• Genome accession: NZ_LR822029
• Coordinates: 1466281..1467600 (-)
• Length: 439 a.a.
• NCBI ID: WP_014607993.1
• Uniprot ID: A0AAN1ZSQ9
• Organism: Streptococcus thermophilus isolate STH_CIRM_1035
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	1467717..1468892	1466281..1467600	flank	117

Gene organization within MGE regions

Location: 1466281..1468892

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H1W89_RS07670 (STHERMO_1691)	comFA/cflA	1466281..1467600 (-)	1320	WP_014607993.1	DEAD/DEAH box helicase	Machinery gene
H1W89_RS07675 (STHERMO_1692)	-	1467717..1468892 (+)	1176	WP_159341049.1	IS256-like element IS1191 family transposase	-

Sequence

Protein

• Length: 439 a.a.
• Molecular weight: 50524.63 Da
• Isoelectric Point: 9.5841

>NTDB_id=1131420 H1W89_RS07670 WP_014607993.1 1466281..1467600(-) (comFA/cflA) [Streptococcus thermophilus isolate STH_CIRM_1035]
MIPKEYYGRLFTKEQLPVDYLSEAVKLESMIKVDKKLRCKRCYSRIEEDWQLPNGQYYCRACIVFGRNQEGKELYYFPSE
KSEVDFPVLKWSGKLTPYQNEVSEKLLKTYKNQKHSLVHAVTGAGKTEMIYNIVAYVLENKNRVVIASPRVDVCRELFLR
MQKDFTCSISLLHADSEPYDGSPLVIATTHQLLKFYHSFDLIIVDEVDAFPFVGNVMLNHAVKQAKTETGRYIYLTATST
LALEEQVRLGAIEKHHLASRFHGNPLVLPCFFWQGRLQKSLTSDKLPRPLIHQIKKQRKSNFPLLIFFPNIALGEKFSIT
LKKYLPTENIAFVSSKSEERSTIVEKFRKKELSILVTTTILERGVTFSQVDVFVCMANHHLYTSSSLIQIGGRVGRSPER
PTGKLYFFHEGLSKSMLQCRKEINAMNKKGGFENEVSTM

Nucleotide

• Length: 1320 bp

>NTDB_id=1131420 H1W89_RS07670 WP_014607993.1 1466281..1467600(-) (comFA/cflA) [Streptococcus thermophilus isolate STH_CIRM_1035]
ATGATACCTAAAGAATATTATGGACGACTATTTACGAAAGAACAGTTACCAGTGGATTATCTCTCAGAGGCTGTAAAATT
AGAAAGTATGATAAAGGTTGATAAAAAACTTAGATGTAAAAGATGTTATAGTCGAATAGAGGAAGATTGGCAATTACCGA
ATGGTCAGTATTATTGTAGAGCGTGTATTGTCTTTGGTCGAAACCAAGAAGGAAAAGAACTCTATTACTTTCCCTCAGAA
AAATCAGAAGTAGATTTTCCTGTCTTGAAATGGTCAGGAAAACTGACTCCTTATCAAAATGAGGTCTCGGAAAAGCTTTT
AAAGACTTATAAAAATCAAAAACACAGTCTTGTTCATGCAGTGACTGGTGCTGGCAAGACAGAGATGATTTATAATATTG
TTGCCTATGTTCTTGAAAATAAAAATCGTGTCGTCATCGCAAGTCCCCGAGTTGATGTTTGTCGAGAATTGTTTCTACGC
ATGCAGAAAGATTTTACTTGTAGTATTTCTCTGCTTCATGCTGATAGTGAACCATATGATGGTAGTCCGCTCGTTATAGC
TACCACTCATCAATTACTAAAATTTTATCATAGCTTTGACTTGATTATTGTTGACGAAGTTGATGCCTTTCCATTTGTAG
GGAATGTCATGTTAAATCATGCTGTTAAACAGGCAAAGACGGAAACAGGCCGGTATATTTACTTAACAGCAACTTCTACA
TTAGCTTTAGAAGAGCAAGTGCGCCTTGGAGCTATAGAAAAGCATCACCTTGCTAGTCGTTTCCACGGAAATCCTTTAGT
CCTTCCTTGTTTCTTTTGGCAAGGAAGGTTACAAAAGTCGTTGACGAGCGATAAGCTTCCAAGGCCTCTAATTCACCAGA
TTAAGAAGCAGCGTAAATCAAATTTTCCTCTATTAATCTTTTTCCCCAATATAGCATTAGGTGAAAAGTTTAGTATTACC
CTAAAAAAATATCTCCCTACTGAAAACATAGCCTTTGTTTCATCAAAAAGCGAGGAGCGTTCAACCATCGTAGAGAAATT
CCGAAAAAAAGAATTGTCAATCTTAGTGACGACAACTATTCTCGAACGTGGTGTTACCTTTTCACAAGTAGATGTTTTTG
TTTGTATGGCAAATCATCACTTATATACTAGTTCGAGTCTTATTCAGATTGGTGGTAGGGTGGGGCGTTCGCCCGAGAGA
CCTACAGGGAAACTCTATTTCTTTCATGAAGGATTATCTAAATCAATGTTGCAATGTCGGAAAGAAATAAATGCAATGAA
TAAAAAAGGAGGGTTTGAAAATGAAGTGTCTACTATGTAA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (94-206)

• Helicase conserved C-terminal domain (Helicase_C) (290-396)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA/cflA	Streptococcus pneumoniae TIGR4	54.988	98.178	0.54
comFA/cflA	Streptococcus pneumoniae Rx1	54.988	98.178	0.54
comFA/cflA	Streptococcus pneumoniae D39	54.988	98.178	0.54
comFA/cflA	Streptococcus pneumoniae R6	54.988	98.178	0.54
comFA/cflA	Streptococcus mitis NCTC 12261	54.651	97.95	0.535
comFA/cflA	Streptococcus mitis SK321	53.721	97.95	0.526
comFA	Lactococcus lactis subsp. cremoris KW2	46.19	95.672	0.442
comFA	Latilactobacillus sakei subsp. sakei 23K	37.011	99.089	0.367