Detailed information NTG1128212

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: E5P2_RS21980
• Genome accession: NZ_LR594666
• Coordinates: 4539694..4540860 (+)
• Length: 388 a.a.
• NCBI ID: WP_162569272.1
• Uniprot ID: A0A8B6LR53
• Organism: Variovorax sp. SRS16
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4534694..4545860

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5P2_RS21955 (G3W92_RS21940)	pxpB	4534946..4535599 (-)	654	WP_162570606.1	5-oxoprolinase subunit PxpB	-
E5P2_RS21960 (G3W92_RS21945)	coaD	4535602..4536105 (-)	504	WP_162569268.1	pantetheine-phosphate adenylyltransferase	-
E5P2_RS21965 (G3W92_RS21950)	rsmD	4536151..4536750 (-)	600	WP_232080930.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
E5P2_RS21970 (G3W92_RS21955)	-	4536843..4538192 (-)	1350	WP_162569270.1	pitrilysin family protein	-
E5P2_RS21975 (G3W92_RS21960)	-	4538207..4539664 (-)	1458	WP_162569271.1	pitrilysin family protein	-
E5P2_RS21980 (G3W92_RS21965)	pilA	4539694..4540860 (+)	1167	WP_162569272.1	signal recognition particle-docking protein FtsY	Machinery gene
E5P2_RS21985 (G3W92_RS21970)	-	4540872..4541198 (-)	327	WP_162569273.1	FHA domain-containing protein	-
E5P2_RS21990 (G3W92_RS21975)	-	4541406..4541867 (+)	462	WP_162569274.1	Mpo1-like protein	-
E5P2_RS21995 (G3W92_RS21980)	rpoH	4542004..4542945 (+)	942	WP_162569275.1	RNA polymerase sigma factor RpoH	-
E5P2_RS22000 (G3W92_RS21985)	-	4542998..4543618 (-)	621	WP_162569276.1	SCO family protein	-
E5P2_RS22005 (G3W92_RS21990)	cyoE	4543626..4544531 (-)	906	WP_162569277.1	heme o synthase	-
E5P2_RS22010 (G3W92_RS21995)	-	4544528..4545715 (-)	1188	WP_162569278.1	COX15/CtaA family protein	-

Sequence

Protein

• Length: 388 a.a.
• Molecular weight: 40957.04 Da
• Isoelectric Point: 5.5306

>NTDB_id=1128212 E5P2_RS21980 WP_162569272.1 4539694..4540860(+) (pilA) [Variovorax sp. SRS16]
MFSFFKKKPPVEVAATPQPEPAPAPAPTEARVEPPAPAKSVFSPSSWFGSKPAPTEPVVASPPVPAPEPVFVAPPAPQAP
VAAAPEPEPERSSWLGKLRTGLRKTGSTITAAFVNAQIDDALYEELEEALLMADTGVKATEHLLEDLRRRVRSNMATEAS
QVKGLLADAITDLLRPLEKALVIGEHTPTVIMVAGVNGAGKTTSIGKLTKHLANEGASVLLAAADTFRAAAREQLLVWAD
RNTVEIVSQEGGDPAAVSFDAVNAGKARGKDVVLVDTAGRLPTQLHLMDELKKIKRVVNKADATAPHEVLLVIDGNTGQN
ALAQVRAFDETLGLTGLVVTKLDGTAKGGVLCAIARERPIPVYFIGVGEKLEDLETFKAREFAQALLG

Nucleotide

• Length: 1167 bp

>NTDB_id=1128212 E5P2_RS21980 WP_162569272.1 4539694..4540860(+) (pilA) [Variovorax sp. SRS16]
ATGTTCAGTTTCTTCAAGAAAAAACCCCCAGTCGAAGTTGCCGCAACGCCGCAGCCCGAGCCCGCACCGGCACCTGCGCC
GACCGAGGCTCGCGTCGAGCCGCCGGCGCCCGCCAAGTCGGTTTTTTCACCGTCCAGCTGGTTCGGGAGCAAGCCGGCAC
CGACCGAGCCGGTGGTGGCGTCGCCGCCTGTACCCGCACCCGAACCTGTCTTTGTTGCACCGCCCGCGCCACAGGCGCCG
GTCGCCGCTGCACCCGAGCCCGAACCCGAGCGCAGCAGCTGGCTCGGCAAGCTCAGGACCGGCCTGCGCAAGACCGGCAG
CACCATCACCGCCGCCTTCGTCAATGCGCAGATCGACGACGCGCTGTACGAAGAGCTCGAAGAGGCGCTGCTGATGGCCG
ATACCGGGGTCAAGGCCACGGAACACCTGCTCGAAGACTTGCGGCGCCGAGTCCGAAGCAACATGGCGACCGAGGCCTCC
CAGGTGAAAGGGCTGCTCGCCGATGCGATCACGGATCTGCTGCGGCCGCTGGAAAAGGCATTGGTCATCGGCGAGCACAC
GCCGACCGTGATCATGGTGGCCGGCGTCAACGGTGCCGGCAAGACCACGTCCATCGGCAAGCTCACCAAGCATCTGGCCA
ACGAAGGCGCGTCGGTGCTGCTGGCCGCGGCCGACACCTTCCGCGCGGCGGCGCGCGAGCAGCTGCTGGTCTGGGCCGAT
CGCAACACCGTGGAGATCGTGAGCCAGGAAGGCGGCGATCCCGCGGCAGTGAGCTTCGATGCGGTCAACGCGGGCAAGGC
GCGCGGCAAGGACGTGGTGCTGGTCGACACGGCCGGCCGGCTGCCGACCCAGCTCCACCTGATGGACGAGCTGAAGAAGA
TCAAGCGCGTGGTCAACAAGGCCGACGCCACGGCGCCGCATGAAGTCCTGCTGGTGATCGACGGCAATACCGGCCAGAAC
GCGCTGGCGCAGGTCAGAGCCTTCGACGAGACCCTGGGCCTGACGGGCCTGGTCGTCACCAAGCTCGATGGCACGGCCAA
GGGCGGTGTCCTGTGCGCCATCGCCCGCGAACGGCCGATCCCGGTCTATTTCATCGGCGTGGGCGAGAAGCTGGAAGACC
TCGAGACCTTCAAGGCGCGGGAGTTCGCCCAGGCGCTGCTGGGGTAG

Domains

Predicted by InterproScan.

• SRP54-type protein, GTPase domain (SRP54) (188-388)

• SRP54-type protein, helical bundle domain (SRP54_N) (107-169)

3D structure

• AlphaFold DB: A0A8B6LR53

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	47.596	100	0.51