Detailed information NTG0112679

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSYRB_RS14470
• Genome accession: NZ_CP005969
• Coordinates: 3407099..3408268 (-)
• Length: 389 a.a.
• NCBI ID: WP_003400837.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae B301D
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3402099..3413268

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSYRB_RS14455 (PsyrB_14800)	-	3403383..3404447 (+)	1065	WP_003400842.1	microcin C ABC transporter permease YejB	-
PSYRB_RS14460 (PsyrB_14805)	-	3404447..3405469 (+)	1023	WP_003400841.1	ABC transporter permease	-
PSYRB_RS14465 (PsyrB_14810)	-	3405471..3407048 (+)	1578	WP_003400838.1	ABC transporter ATP-binding protein	-
PSYRB_RS14470 (PsyrB_14815)	pilU	3407099..3408268 (-)	1170	WP_003400837.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSYRB_RS14475 (PsyrB_14820)	-	3408392..3408667 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
PSYRB_RS14480 (PsyrB_14825)	pgm	3408742..3410388 (-)	1647	WP_011268077.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PSYRB_RS14485 (PsyrB_14830)	-	3410552..3412879 (-)	2328	WP_032657338.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42577.65 Da
• Isoelectric Point: 6.0641

>NTDB_id=112679 PSYRB_RS14470 WP_003400837.1 3407099..3408268(-) (pilU) [Pseudomonas syringae pv. syringae B301D]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETFKPGEVAVIAQGLMDEEQKLEFQRELEMNLAVSMAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDSAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGAGSLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=112679 PSYRB_RS14470 WP_003400837.1 3407099..3408268(-) (pilU) [Pseudomonas syringae pv. syringae B301D]
ATGGATTTCCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTACCTCTCCACCGGAGCGCCACCGTG
CGCCAAGTTCAACGGTGTGCTCAAGCCACTGGGCAGCGAAACCTTCAAGCCGGGGGAGGTCGCAGTCATTGCCCAGGGGC
TGATGGACGAAGAACAGAAGCTCGAGTTTCAACGCGAACTGGAGATGAACCTCGCCGTTTCGATGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTTATGCAGCGCAACGAAGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATCCCGCGTTTCGA
AGACCTGTTCCTGCCGCCCGTGCTGCTCGACGTGATCATGGAAAAGCGCGGTCTGGTGCTGTTTGTCGGGGCCACCGGTT
CGGGCAAGTCGACCTCATTGGCGGCGCTGATCGACTACCGTAACCGCAACGCCAGCGGCCATATCATCACCATTGAGGAC
CCGGTCGAGTTCATCCATCGCCACAAGAAATCGATCGTCAACCAGCGCGAAGTCGGGGTCGATACCCGCAGCTTCCATGC
GGCATTGAAAAACACGCTGCGCCAGGCGCCGGATGTGATTCTGATCGGCGAGATTCGTGATCGCGAGACCATGGAGCACG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCACGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAATTTCTTCCCGGAAGAGCGTCGCGCGCAGTTACTGCATGACCTGGGCAACAACCTCAAGGCATTCGTCTCCCAGCG
GCTGGTCAGAACCCCGGATGGCAAGCGCCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATCC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAGTCCGGCAGCCTCGGTATGCAGACGTTCGACTCGGCGCTG
TTCAATCTGGCCGTCGAAGGGGCGATCAGCGAAGAAGAAGCCCTCAAGAACGCCGACTCGCAGAACAACGTGCGCCTGCG
CCTCAAACTGCACAGTGAAGGCGGCGCCGGGAGCCTCACCACACCGCCGCCGGCCCCGACCGGCAGCAGCACGGCAAGCA
CCGCCGAGTGGGGGCTGGTCGATGATGACGCGCCGGGTCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.287	93.059	0.542
pilU	Vibrio cholerae strain A1552	51.105	93.059	0.476
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas stutzeri DSM 10701	41.108	88.175	0.362
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362