Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   FGK83_RS04085 Genome accession   NZ_LR590465
Coordinates   808042..808581 (-) Length   179 a.a.
NCBI ID   WP_105876923.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC8468     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 803042..813581
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGK83_RS04055 (NCTC8468_00804) fdx 803457..803798 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  FGK83_RS04060 (NCTC8468_00805) iscX 803798..803992 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  FGK83_RS04065 (NCTC8468_00806) - 804043..804657 (-) 615 WP_050847273.1 YfgM family protein -
  FGK83_RS04070 (NCTC8468_00807) hisS 804675..805946 (-) 1272 WP_138171747.1 histidine--tRNA ligase -
  FGK83_RS04075 (NCTC8468_00808) ispG 805956..807062 (-) 1107 WP_048954971.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  FGK83_RS04080 (NCTC8468_00809) - 807071..807970 (-) 900 WP_138171748.1 RodZ domain-containing protein -
  FGK83_RS04085 (NCTC8468_00810) pilF/pilF2 808042..808581 (-) 540 WP_105876923.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  FGK83_RS04090 (NCTC8468_00811) - 808682..809833 (-) 1152 WP_005666614.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  FGK83_RS04095 (NCTC8468_00812) - 810080..810958 (+) 879 WP_005649130.1 D-alanyl-D-alanine carboxypeptidase family protein -
  FGK83_RS04100 (NCTC8468_00813) - 811320..812201 (+) 882 WP_005669100.1 metal ABC transporter substrate-binding protein -
  FGK83_RS04105 (NCTC8468_00814) - 812201..813121 (+) 921 WP_105879971.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20573.28 Da        Isoelectric Point: 9.2101

>NTDB_id=1126754 FGK83_RS04085 WP_105876923.1 808042..808581(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC8468]
MQTISKQLSAVIFPFIFSACVSQSASSLDHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVKLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYRQADTFENIALCAYSAKKMNIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1126754 FGK83_RS04085 WP_105876923.1 808042..808581(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC8468]
ATGCAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTCATTTTTTCTGCCTGCGTTTCACAATCAGCATCTAG
CTTAGATCATCAAGCTGCCGCCAAAGCGCGAGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGACAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAAACTTAATCATAAACAAGGCGATGTACATAATAA
TTTTGGTACATTTCTATGTAGTCAAAAGAAATTTGAGCAAGCCCAGCAACAATTTGAATTAGCCCTTAATTCGCCGAATT
ATTATCGTCAAGCAGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGAAAAAAATGAATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(73-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

95.531

100

0.955
  pilF Glaesserella parasuis strain SC1401

45.81

100

0.458

Multiple sequence alignment