Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   E0F32_RS04625 Genome accession   NZ_LR216050
Coordinates   849509..851311 (+) Length   600 a.a.
NCBI ID   WP_024477773.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 844509..856311
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0F32_RS04605 (SAMEA3431333_00861) rnr 844804..847158 (+) 2355 WP_000654886.1 ribonuclease R -
  E0F32_RS04610 (SAMEA3431333_00862) smpB 847121..847588 (+) 468 WP_001051750.1 SsrA-binding protein SmpB -
  E0F32_RS04615 (SAMEA3431333_00863) tehB 847604..848464 (+) 861 WP_000413762.1 SAM-dependent methyltransferase TehB -
  E0F32_RS04620 (SAMEA3431333_00864) coiA 848537..849490 (+) 954 WP_000495911.1 competence protein CoiA Machinery gene
  E0F32_RS04625 (SAMEA3431333_00865) pepF 849509..851311 (+) 1803 WP_024477773.1 oligoendopeptidase F Regulator
  E0F32_RS04630 (SAMEA3431333_00866) - 851313..852026 (+) 714 WP_000230399.1 O-methyltransferase -
  E0F32_RS04635 (SAMEA3431333_00867) prsA 852093..853034 (+) 942 WP_000727935.1 peptidylprolyl isomerase PrsA -
  E0F32_RS04640 (SAMEA3431333_00868) - 853151..853492 (-) 342 Protein_876 transposase -
  E0F32_RS04650 - 853905..854000 (+) 96 Protein_877 IS630 family transposase -
  E0F32_RS04655 (SAMEA3431333_00870) - 854253..854873 (-) 621 WP_000780815.1 histidine phosphatase family protein -
  E0F32_RS04660 (SAMEA3431333_00871) - 854873..855355 (-) 483 WP_001085886.1 aminoacyl-tRNA deacylase -
  E0F32_RS04665 (SAMEA3431333_00872) - 855355..856197 (-) 843 WP_000954117.1 DUF368 domain-containing protein -

Sequence

Protein


Download         Length: 600 a.a.        Molecular weight: 69890.79 Da        Isoelectric Point: 4.5469

>NTDB_id=1126111 E0F32_RS04625 WP_024477773.1 849509..851311(+) (pepF) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYEGHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKND
QDTREAKYQEYYAKAMTLYSQLDQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQALYATYEQFQHTYAKTLQTNVKVQ
NYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRKHLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYNFTYQEALK
KAEDALAVLGEDYLSRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSMHSSYTRET
QPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFRGTVFRQTQFAEFEHAIHQADQNGEVLTSDF
LNKLYADLNQEYYGLSKEDNPEIQYEWARIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPL
NVMRKAGVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA

Nucleotide


Download         Length: 1803 bp        

>NTDB_id=1126111 E0F32_RS04625 WP_024477773.1 849509..851311(+) (pepF) [Streptococcus pneumoniae strain GPSC10 substr. ST2013 isolate GPS_US_PATH396-sc-2296505]
ATGGTATTACAAAGAAATGAAATAAATGAAAAAGATACATGGGATCTATCAACGATCTACCCAACTGACCAGGCTTGGGA
AGAAGCCTTAAAAGATTTAACAGAACAATTGGAGACAGTAGCCCAGTATGAAGGCCATCTCTTGGATAGTGCGGATAACC
TACTAGAAATCACTGAATTTTCTCTTGAAATGGAACGCCAGATAGAGAAGCTTTACGTTTATGCTCATATGAAGAATGAC
CAGGATACACGTGAAGCTAAGTATCAAGAGTACTATGCCAAGGCCATGACACTCTACAGCCAGTTAGACCAAGCCTTTTC
ATTCTATGAGCCTGAATTTATGGAGATTAGCGAAAAGCAGTATGCTGACTTTTTAGAAGCTCAACCAAAGCTGCAGGTTT
ATCAACACTATTTTGACAAGCTCTTGCAAGGCAAGGATCACGTTCTTTCACAACGTGAAGAAGAATTATTGGCTGGAGCT
GGAGAAATCTTTGGTTCAGCAAGTGAAACCTTCGCTATCTTGGACAATGCGGATATTGTGTTCCCTTATGTCCTAGACGA
TGATGGTAAAGAAGTTCAGCTATCTCATGGGACTTACACACGTTTGATGGAGTCTAAAAAACGTGAGGTTCGCCGTGGTG
CCTATCAAGCTCTTTATGCGACTTACGAACAATTCCAACACACCTATGCCAAAACCTTGCAAACCAATGTTAAGGTGCAA
AATTACCGTGCTAAAGTTCGTAACTACAAGAGTGCTCGTCATGCAGCCCTCGCAGCGAATTTTGTTCCAGAAAGTGTTTA
TGACAATTTGGTAGCAGCAGTTCGCAAGCATTTGCCACTCTTACATCGCTATCTTGAGCTTCGTTCAAAAATCTTGGGGA
TTTCAGATCTCAAGATGTACGATGTCTACACACCGCTTTCATCTGTTGAATACAATTTTACCTACCAAGAAGCCTTGAAA
AAAGCAGAAGATGCTTTGGCAGTCTTGGGTGAGGATTACTTGAGCCGTGTCAAACGTGCCTTCAGCGAGCGTTGGATTGA
TGTTTACGAAAATCAAGGCAAGCGTTCAGGTGCCTACTCTGGTGGTTCTTACGATACCAATGCCTTTATGCTTCTCAACT
GGCAGGACAATCTGGACAATCTCTTTACTCTTGTTCATGAAACAGGTCACAGTATGCATTCAAGCTATACTCGTGAAACT
CAGCCTTATGTTTACGGAGATTACTCTATCTTTTTGGCTGAGATTGCCTCAACTACCAATGAAAATATCTTGACGGAGAA
ATTATTGGAAGAAGTGGAAGACGACGCAACACGCTTTGCTATTCTCAATAACTTCCTAGATGGTTTCCGTGGAACAGTTT
TCCGCCAAACTCAATTTGCTGAGTTTGAACACGCCATTCACCAAGCAGATCAAAATGGGGAGGTCTTGACAAGCGATTTC
CTAAATAAACTCTACGCAGACTTGAACCAAGAGTATTATGGTTTGAGTAAGGAAGACAATCCTGAAATCCAATACGAGTG
GGCTCGCATTCCACACTTCTACTATAACTACTATGTATATCAATATTCAACTGGCTTTGCGGCCGCCTCAGCCTTGGCTG
AAAAAATTGTCCATGGTAGTCAAGAAGACCGTGACCGCTATATCGACTACCTCAAGGCAGGTAAGTCGGACTATCCACTT
AATGTCATGAGAAAAGCTGGTGTTGATATGGAGAAGGAAGACTACCTCAACGATGCCTTTGCAGTCTTTGAACGCCGTTT
AAATGAGTTTGAAGCCCTTGTTGAAAAATTAGGATTGGCATAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(112-181)

Peptidase family M3 (Peptidase_M3)

(202-581)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

67.45

99.333

0.67

Multiple sequence alignment