Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   EL300_RS06620 Genome accession   NZ_LR134532
Coordinates   1425554..1426099 (-) Length   181 a.a.
NCBI ID   WP_005752449.1    Uniprot ID   -
Organism   Pasteurella multocida strain NCTC10323     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1420554..1431099
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL300_RS06595 (NCTC10323_01344) - 1420764..1421201 (-) 438 WP_005725139.1 methyltransferase family protein -
  EL300_RS06600 (NCTC10323_01345) - 1421414..1422025 (-) 612 WP_005725137.1 YfgM family protein -
  EL300_RS06605 (NCTC10323_01346) hisS 1422044..1423315 (-) 1272 WP_005752452.1 histidine--tRNA ligase -
  EL300_RS06610 (NCTC10323_01347) ispG 1423345..1424448 (-) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EL300_RS06615 (NCTC10323_01348) - 1424475..1425431 (-) 957 WP_005725132.1 helix-turn-helix domain-containing protein -
  EL300_RS06620 (NCTC10323_01349) pilF/pilF2 1425554..1426099 (-) 546 WP_005752449.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL300_RS06625 (NCTC10323_01350) - 1426139..1427323 (-) 1185 WP_005752447.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL300_RS06630 (NCTC10323_01351) - 1427504..1428757 (-) 1254 WP_005752439.1 AI-2E family transporter -
  EL300_RS06635 (NCTC10323_01352) glnB 1428758..1429096 (-) 339 WP_005725112.1 nitrogen regulatory protein P-II -
  EL300_RS06640 (NCTC10323_01353) mog 1429141..1429719 (-) 579 WP_005737641.1 molybdopterin adenylyltransferase -
  EL300_RS06645 (NCTC10323_01354) - 1429745..1430674 (-) 930 WP_005725108.1 phosphatidate cytidylyltransferase -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20911.47 Da        Isoelectric Point: 7.8913

>NTDB_id=1124090 EL300_RS06620 WP_005752449.1 1425554..1426099(-) (pilF/pilF2) [Pasteurella multocida strain NCTC10323]
MTRVFQVWIASIFSLFLSACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYLYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGTFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1124090 EL300_RS06620 WP_005752449.1 1425554..1426099(-) (pilF/pilF2) [Pasteurella multocida strain NCTC10323]
ATGACGCGCGTTTTTCAAGTATGGATAGCAAGCATTTTTTCTCTTTTTCTTTCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCAGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATCTCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTACTTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCCGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCTAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50.568

97.238

0.492
  pilF Glaesserella parasuis strain SC1401

44.385

100

0.459

Multiple sequence alignment