Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL265_RS06840 Genome accession   NZ_LR134515
Coordinates   1481381..1481929 (-) Length   182 a.a.
NCBI ID   WP_005601803.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain NCTC10976     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1476381..1486929
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL265_RS06825 (NCTC10976_01378) - 1476875..1477396 (+) 522 WP_005598290.1 SprT family zinc-dependent metalloprotease -
  EL265_RS06830 (NCTC10976_01379) - 1477501..1478787 (+) 1287 WP_005601799.1 NAD(P)/FAD-dependent oxidoreductase -
  EL265_RS06835 (NCTC10976_01380) - 1478979..1481306 (-) 2328 WP_005601801.1 Tex family protein -
  EL265_RS06840 (NCTC10976_01381) pilF 1481381..1481929 (-) 549 WP_005601803.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL265_RS06845 (NCTC10976_01382) - 1481983..1483164 (-) 1182 WP_005601805.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL265_RS06865 (NCTC10976_01386) gltX 1483942..1485381 (+) 1440 WP_005601815.1 glutamate--tRNA ligase -
  EL265_RS06875 (NCTC10976_01388) yefM 1485719..1485970 (+) 252 WP_005598318.1 YoeB-YefM toxin-antitoxin system antitoxin YefM -
  EL265_RS06880 (NCTC10976_01389) - 1485967..1486227 (+) 261 WP_005605064.1 Txe/YoeB family addiction module toxin -
  EL265_RS06885 (NCTC10976_01390) - 1486386..1486553 (-) 168 WP_005598320.1 Trm112 family protein -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 20603.14 Da        Isoelectric Point: 6.6300

>NTDB_id=1123693 EL265_RS06840 WP_005601803.1 1481381..1481929(-) (pilF) [Actinobacillus pleuropneumoniae strain NCTC10976]
MNLAKFLRNLSACMLLSVLFGCSSQLPTSQTTFNRSEAVKARINLALAYLDQHDLPKAKENIDKALSHDQNDYLPHSVLA
YYYQQIGDQVHAEQAYQQALKSSDNRPDVLNNYGTFLCKQAKFDQAYQQFEQAVQSEQPYYHQADSLENIVLCAKQEPNL
AKANEALSQLEKLDKSRAAALR

Nucleotide


Download         Length: 549 bp        

>NTDB_id=1123693 EL265_RS06840 WP_005601803.1 1481381..1481929(-) (pilF) [Actinobacillus pleuropneumoniae strain NCTC10976]
ATGAATCTTGCAAAATTTTTGCGAAATTTGAGCGCTTGTATGCTGTTATCCGTGTTGTTCGGTTGTTCTTCTCAGCTTCC
AACCTCCCAAACTACCTTTAATCGTTCGGAAGCGGTTAAAGCCCGTATTAATTTAGCCTTAGCGTATCTCGATCAACATG
ATTTACCCAAAGCGAAAGAGAATATTGATAAAGCGCTAAGCCACGATCAAAACGATTATTTGCCTCACTCTGTTTTAGCT
TATTACTATCAGCAAATCGGTGATCAAGTTCATGCGGAACAGGCTTATCAGCAAGCATTGAAATCAAGTGATAATCGTCC
GGATGTCTTAAATAATTACGGAACTTTTCTGTGTAAACAAGCCAAATTTGATCAGGCATACCAACAATTTGAGCAAGCGG
TGCAAAGCGAACAACCTTATTATCATCAAGCCGATAGCCTCGAAAATATTGTGTTATGTGCCAAACAAGAGCCGAATTTA
GCTAAAGCAAATGAAGCGTTAAGCCAATTAGAAAAGCTGGATAAATCAAGAGCGGCGGCACTACGCTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

65.957

100

0.681
  pilF/pilF2 Haemophilus influenzae Rd KW20

46.784

93.956

0.44

Multiple sequence alignment