Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL242_RS10140 Genome accession   NZ_LR134495
Coordinates   2080964..2081518 (-) Length   184 a.a.
NCBI ID   WP_006251773.1    Uniprot ID   -
Organism   Mannheimia haemolytica strain NCTC10643     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2075964..2086518
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL242_RS10125 (NCTC10643_02014) sppA 2076049..2077905 (-) 1857 WP_126302485.1 signal peptide peptidase SppA -
  EL242_RS10130 (NCTC10643_02015) - 2078007..2078570 (+) 564 WP_126302486.1 nitroreductase family protein -
  EL242_RS10135 (NCTC10643_02016) - 2078640..2080964 (-) 2325 WP_126302487.1 Tex family protein -
  EL242_RS10140 (NCTC10643_02017) pilF 2080964..2081518 (-) 555 WP_006251773.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL242_RS10145 (NCTC10643_02018) - 2081733..2082104 (+) 372 WP_126302488.1 type II toxin-antitoxin system RelE/ParE family toxin -
  EL242_RS10150 (NCTC10643_02019) - 2082088..2082390 (+) 303 WP_126302489.1 helix-turn-helix domain-containing protein -
  EL242_RS10155 (NCTC10643_02020) - 2082429..2083589 (-) 1161 WP_126302490.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL242_RS10160 (NCTC10643_02021) - 2083851..2084747 (+) 897 WP_006251769.1 metal ABC transporter substrate-binding protein -
  EL242_RS10165 (NCTC10643_02022) - 2084760..2085503 (+) 744 WP_126302491.1 metal ABC transporter ATP-binding protein -
  EL242_RS10170 (NCTC10643_02023) - 2085548..2086357 (-) 810 WP_126302492.1 metal ABC transporter permease -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 20976.90 Da        Isoelectric Point: 7.0805

>NTDB_id=1123272 EL242_RS10140 WP_006251773.1 2080964..2081518(-) (pilF) [Mannheimia haemolytica strain NCTC10643]
MFAKFFPKMTACILIFLLAACSSLPPSSSEVEFNRSEAVKARINLALAYLEQNDFPKAKQNIDKALAHDEKDYLPYSVLA
YYYQQTGDAENAEKAYQTALKLSQNRPDVLNNYGTFLCKQGQFQQAYQQFEQAVKSKAPYYHQADSLENMVLCAKKEPNL
QKIAETLTELEKLDKPRANLLKEN

Nucleotide


Download         Length: 555 bp        

>NTDB_id=1123272 EL242_RS10140 WP_006251773.1 2080964..2081518(-) (pilF) [Mannheimia haemolytica strain NCTC10643]
ATGTTTGCAAAATTTTTTCCAAAAATGACCGCTTGTATTCTGATTTTTTTATTAGCGGCTTGTTCTTCTCTTCCCCCTTC
TTCCTCTGAAGTCGAATTTAACCGCTCGGAAGCGGTAAAAGCCCGTATTAATTTGGCTTTAGCTTATCTGGAGCAAAATG
ATTTCCCAAAAGCTAAGCAGAATATTGATAAAGCCTTGGCTCACGATGAAAAAGATTACCTCCCTTATTCGGTGCTTGCC
TATTATTACCAACAAACAGGTGATGCTGAAAATGCCGAAAAAGCCTACCAAACGGCACTGAAATTGAGTCAAAACCGCCC
TGATGTGCTGAATAATTACGGCACTTTTTTATGTAAACAGGGTCAATTTCAACAGGCTTATCAGCAATTTGAGCAGGCTG
TCAAAAGCAAAGCTCCTTATTATCACCAAGCAGATAGCTTAGAAAATATGGTGTTATGTGCGAAAAAAGAGCCGAATCTG
CAAAAAATTGCCGAAACATTGACCGAGCTTGAGAAATTGGATAAACCTCGAGCAAATTTATTGAAGGAAAACTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(95-126)

Tetratricopeptide repeat (TPR_8)

(40-67)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

63.636

100

0.647
  pilF/pilF2 Haemophilus influenzae Rd KW20

47.953

92.935

0.446

Multiple sequence alignment