Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   EL230_RS03535 Genome accession   NZ_LR134490
Coordinates   700349..700888 (-) Length   179 a.a.
NCBI ID   WP_012054975.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC11873     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 695349..705888
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL230_RS03505 (NCTC11873_00694) fdx 695752..696093 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  EL230_RS03510 (NCTC11873_00695) iscX 696093..696287 (+) 195 WP_005659903.1 Fe-S cluster assembly protein IscX -
  EL230_RS03515 (NCTC11873_00696) - 696338..696952 (-) 615 WP_112109782.1 YfgM family protein -
  EL230_RS03520 (NCTC11873_00697) hisS 696970..698241 (-) 1272 WP_042611740.1 histidine--tRNA ligase -
  EL230_RS03525 (NCTC11873_00698) ispG 698251..699357 (-) 1107 WP_005649145.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EL230_RS03530 (NCTC11873_00699) - 699366..700277 (-) 912 WP_012054976.1 helix-turn-helix domain-containing protein -
  EL230_RS03535 (NCTC11873_00700) pilF/pilF2 700349..700888 (-) 540 WP_012054975.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL230_RS03540 (NCTC11873_00701) - 700989..702140 (-) 1152 WP_005662659.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL230_RS03545 (NCTC11873_00702) - 702387..703265 (+) 879 WP_005659920.1 D-alanyl-D-alanine carboxypeptidase family protein -
  EL230_RS03550 (NCTC11873_00703) - 703631..704512 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  EL230_RS03555 (NCTC11873_00704) - 704512..705432 (+) 921 WP_110434977.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20554.15 Da        Isoelectric Point: 8.2571

>NTDB_id=1123195 EL230_RS03535 WP_012054975.1 700349..700888(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC11873]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDNALQHDKNYYLVHSALAHYYQQ
QGQIENAFREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1123195 EL230_RS03535 WP_012054975.1 700349..700888(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC11873]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGCGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGATAACGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGCAAATAGAAAATGCCTTTCGTGAGTATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCTCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCAGATACATTTGAAAATATTGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.883

100

0.989
  pilF Glaesserella parasuis strain SC1401

44.134

100

0.441

Multiple sequence alignment