Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   EL215_RS10130 Genome accession   NZ_LR134481
Coordinates   2043746..2044288 (-) Length   180 a.a.
NCBI ID   WP_126471926.1    Uniprot ID   A0A3S4VEQ5
Organism   Haemophilus parainfluenzae strain NCTC10665     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2038746..2049288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL215_RS10105 (NCTC10665_02036) - 2039122..2039565 (-) 444 WP_126471918.1 methyltransferase family protein -
  EL215_RS10110 (NCTC10665_02037) - 2039617..2040231 (-) 615 WP_126471920.1 YfgM family protein -
  EL215_RS10115 (NCTC10665_02038) hisS 2040250..2041521 (-) 1272 WP_126471922.1 histidine--tRNA ligase -
  EL215_RS10120 (NCTC10665_02039) ispG 2041531..2042637 (-) 1107 WP_005695590.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EL215_RS10125 (NCTC10665_02040) - 2042656..2043678 (-) 1023 WP_126471924.1 RodZ domain-containing protein -
  EL215_RS10130 (NCTC10665_02041) pilF/pilF2 2043746..2044288 (-) 543 WP_126471926.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL215_RS10135 (NCTC10665_02042) - 2044358..2045506 (-) 1149 WP_049357003.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL215_RS10140 (NCTC10665_02043) - 2045808..2046683 (+) 876 WP_049363744.1 D-alanyl-D-alanine carboxypeptidase family protein -
  EL215_RS10145 (NCTC10665_02044) - 2046837..2047718 (+) 882 WP_126471928.1 metal ABC transporter substrate-binding protein -
  EL215_RS10150 (NCTC10665_02045) - 2047718..2048614 (+) 897 WP_070582499.1 manganese/iron ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20366.99 Da        Isoelectric Point: 6.4774

>NTDB_id=1123048 EL215_RS10130 WP_126471926.1 2043746..2044288(-) (pilF/pilF2) [Haemophilus parainfluenzae strain NCTC10665]
MKLIPINILSAVIFPFVFSACVSQSSVDFNKQQAAKARVELALGYLQQNDFVQAKLNLDKAFEHDDRYYLVHSALAHFYQ
LQGDTEKAKQAYLQAIKLDDKQGDVYNNFGAFLCGQGEFEQAYSQFNAALAAPNYYHQADTYENMALCAFAAKQTDAYQQ
ALEKLRQVDPSRAEKLRSLK

Nucleotide


Download         Length: 543 bp        

>NTDB_id=1123048 EL215_RS10130 WP_126471926.1 2043746..2044288(-) (pilF/pilF2) [Haemophilus parainfluenzae strain NCTC10665]
ATGAAATTAATCCCAATTAACATTCTAAGTGCGGTCATTTTTCCTTTTGTTTTTTCTGCTTGTGTTTCTCAGTCTTCTGT
TGATTTTAATAAACAACAAGCAGCAAAAGCGCGCGTTGAATTGGCATTAGGCTATCTTCAACAAAATGATTTCGTTCAAG
CAAAACTGAATTTAGATAAAGCTTTTGAACATGATGACCGCTATTATCTTGTGCACTCGGCACTTGCGCATTTTTATCAA
TTACAAGGCGATACGGAAAAAGCGAAGCAAGCTTATTTACAGGCGATTAAGCTAGACGATAAGCAAGGCGATGTGTATAA
CAACTTTGGTGCATTTTTATGTGGGCAAGGTGAGTTTGAACAAGCTTATTCACAATTTAATGCAGCACTTGCTGCACCCA
ATTATTATCATCAAGCTGATACTTACGAAAACATGGCACTTTGTGCTTTTGCTGCAAAACAAACCGATGCTTATCAACAG
GCATTGGAGAAATTACGCCAAGTTGATCCTTCTAGAGCGGAAAAGCTCCGTTCACTCAAATAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(74-101)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4VEQ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

66.86

95.556

0.639
  pilF Glaesserella parasuis strain SC1401

49.153

98.333

0.483

Multiple sequence alignment