Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   EL190_RS04185 Genome accession   NZ_LR134395
Coordinates   780612..781151 (-) Length   179 a.a.
NCBI ID   WP_006996133.1    Uniprot ID   -
Organism   Haemophilus aegyptius strain NCTC8134     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 775612..786151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL190_RS04155 (NCTC8134_00849) fdx 776015..776356 (+) 342 WP_006996128.1 ISC system 2Fe-2S type ferredoxin -
  EL190_RS04160 (NCTC8134_00850) iscX 776356..776550 (+) 195 WP_005630102.1 Fe-S cluster assembly protein IscX -
  EL190_RS04165 (NCTC8134_00851) - 776601..777215 (-) 615 WP_006996129.1 YfgM family protein -
  EL190_RS04170 (NCTC8134_00852) hisS 777233..778504 (-) 1272 WP_006996130.1 histidine--tRNA ligase -
  EL190_RS04175 (NCTC8134_00853) ispG 778514..779620 (-) 1107 WP_006996131.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EL190_RS04180 (NCTC8134_00854) - 779629..780540 (-) 912 WP_006996132.1 helix-turn-helix domain-containing protein -
  EL190_RS04185 (NCTC8134_00855) pilF/pilF2 780612..781151 (-) 540 WP_006996133.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL190_RS04190 (NCTC8134_00856) - 781252..782403 (-) 1152 WP_013527479.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL190_RS04195 (NCTC8134_00857) - 782650..783528 (+) 879 WP_005662660.1 D-alanyl-D-alanine carboxypeptidase family protein -
  EL190_RS10570 - 783520..783645 (-) 126 WP_257005528.1 hypothetical protein -
  EL190_RS04200 (NCTC8134_00858) - 783893..784774 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  EL190_RS04205 (NCTC8134_00859) - 784774..785694 (+) 921 WP_006996135.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20531.12 Da        Isoelectric Point: 8.2626

>NTDB_id=1122727 EL190_RS04185 WP_006996133.1 780612..781151(-) (pilF/pilF2) [Haemophilus aegyptius strain NCTC8134]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIALCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1122727 EL190_RS04185 WP_006996133.1 780612..781151(-) (pilF/pilF2) [Haemophilus aegyptius strain NCTC8134]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTCCCTTTTATTTTTTCTGCTTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGCGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAACTTAGATAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCGAATT
ATTATCATCAAGCGGATACATTTGAAAATATCGCGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACT
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGTCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

97.765

100

0.978
  pilF Glaesserella parasuis strain SC1401

45.251

100

0.453

Multiple sequence alignment