Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL148_RS02140 Genome accession   NZ_LR134364
Coordinates   431702..432247 (-) Length   181 a.a.
NCBI ID   WP_064082520.1    Uniprot ID   -
Organism   [Haemophilus] ducreyi strain NCTC11483     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426702..437247
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL148_RS02125 (NCTC11483_00446) dppD 427270..428274 (+) 1005 WP_074648016.1 dipeptide ABC transporter ATP-binding protein -
  EL148_RS02130 (NCTC11483_00447) - 428252..429256 (+) 1005 WP_010944346.1 peptide ABC transporter ATP-binding protein -
  EL148_RS02135 (NCTC11483_00448) - 429313..431631 (-) 2319 WP_074648014.1 Tex family protein -
  EL148_RS02140 (NCTC11483_00449) pilF 431702..432247 (-) 546 WP_064082520.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL148_RS02145 (NCTC11483_00450) - 432286..433467 (-) 1182 WP_064082521.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL148_RS02165 (NCTC11483_00454) gltX 434241..435680 (+) 1440 WP_064082522.1 glutamate--tRNA ligase -
  EL148_RS02175 (NCTC11483_00456) sodA 435934..436575 (-) 642 WP_010944351.1 superoxide dismutase [Mn] -
  EL148_RS02180 (NCTC11483_00457) - 436715..437068 (-) 354 WP_064082523.1 RidA family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20343.02 Da        Isoelectric Point: 8.1148

>NTDB_id=1122294 EL148_RS02140 WP_064082520.1 431702..432247(-) (pilF) [[Haemophilus] ducreyi strain NCTC11483]
MAKILKNITACFTIVMLLGCSSSYTSSSVSTFNRNKIVQARINLALAYLEQHHFAKAKENIDKALQHDAQDYLPYSAQAY
YYQQIGDLTQAANAYQTALTLGNNTPDVLNNYGTFLCHQGKFDLAYQQFEQAIKSQQSYYHQADSLENIILCAKQEKNTA
KITIALQQLTALDKTRADALR

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1122294 EL148_RS02140 WP_064082520.1 431702..432247(-) (pilF) [[Haemophilus] ducreyi strain NCTC11483]
ATGGCAAAAATTTTAAAAAATATAACCGCTTGTTTCACTATTGTTATGCTGTTAGGTTGTTCCTCTTCTTATACTAGCTC
ATCAGTATCCACATTTAACCGAAATAAAATAGTCCAAGCACGCATTAATTTAGCATTAGCTTATCTAGAACAACACCATT
TTGCTAAAGCAAAAGAAAATATTGATAAAGCACTCCAACATGACGCTCAAGATTATTTACCTTATTCAGCACAGGCTTAC
TATTATCAACAAATAGGTGATTTAACTCAAGCCGCCAACGCCTACCAAACAGCACTAACTCTAGGAAATAATACACCTGA
TGTATTAAATAATTACGGTACTTTTTTATGCCATCAAGGTAAATTTGACCTCGCTTATCAGCAATTTGAACAAGCGATAA
AGAGTCAACAATCTTACTATCATCAAGCAGATAGCTTAGAGAATATTATTCTTTGTGCAAAACAAGAGAAAAATACCGCT
AAAATAACTATTGCATTACAACAATTAACAGCACTAGACAAAACACGCGCAGATGCACTACGGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_16)

(77-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Glaesserella parasuis strain SC1401

57.219

100

0.591
  pilF/pilF2 Haemophilus influenzae Rd KW20

43.182

97.238

0.42

Multiple sequence alignment