Detailed information NTG1122136

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: EL160_RS06930
• Genome accession: NZ_LR134351
• Coordinates: 1357704..1358201 (-)
• Length: 165 a.a.
• NCBI ID: WP_001794055.1
• Uniprot ID: A0A7U7EV69
• Organism: Staphylococcus aureus strain NCTC13811
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1352704..1363201

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL160_RS06905 (NCTC13811_01314)	gcvPB	1352866..1354338 (-)	1473	WP_000202178.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
EL160_RS06910 (NCTC13811_01315)	gcvPA	1354331..1355677 (-)	1347	WP_000019697.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
EL160_RS06915 (NCTC13811_01316)	gcvT	1355697..1356788 (-)	1092	WP_000093347.1	glycine cleavage system aminomethyltransferase GcvT	-
EL160_RS06920	-	1356947..1357471 (-)	525	WP_001015125.1	shikimate kinase	-
EL160_RS06925	-	1357461..1357607 (-)	147	WP_001789879.1	hypothetical protein	-
EL160_RS06930 (NCTC13811_01318)	comGF	1357704..1358201 (-)	498	WP_001794055.1	competence type IV pilus minor pilin ComGF	Machinery gene
EL160_RS06935 (NCTC13811_01319)	comGE	1358119..1358418 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
EL160_RS06940 (NCTC13811_01320)	comGD	1358405..1358851 (-)	447	WP_001791207.1	competence type IV pilus minor pilin ComGD	Machinery gene
EL160_RS06945 (NCTC13811_01321)	comGC	1358829..1359140 (-)	312	WP_000472257.1	competence type IV pilus major pilin ComGC	Machinery gene
EL160_RS06950 (NCTC13811_01322)	comGB	1359154..1360224 (-)	1071	WP_000776412.1	competence type IV pilus assembly protein ComGB	Machinery gene
EL160_RS06955 (NCTC13811_01323)	comGA	1360196..1361170 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
EL160_RS06960 (NCTC13811_01324)	-	1361222..1361845 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
EL160_RS06965 (NCTC13811_01325)	-	1361842..1362171 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
EL160_RS06970 (NCTC13811_01326)	-	1362171..1363157 (-)	987	WP_000161314.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19224.98 Da
• Isoelectric Point: 10.2192

>NTDB_id=1122136 EL160_RS06930 WP_001794055.1 1357704..1358201(-) (comGF) [Staphylococcus aureus strain NCTC13811]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLNKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=1122136 EL160_RS06930 WP_001794055.1 1357704..1358201(-) (comGF) [Staphylococcus aureus strain NCTC13811]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAACAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A7U7EV69

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97