Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL147_RS08210 Genome accession   NZ_LR134341
Coordinates   1677871..1679172 (+) Length   433 a.a.
NCBI ID   WP_007891532.1    Uniprot ID   G5K819
Organism   Streptococcus pseudoporcinus strain NCTC13786     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1672871..1684172
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL147_RS08180 (NCTC13786_01644) - 1672971..1673612 (+) 642 WP_007892962.1 response regulator transcription factor -
  EL147_RS08185 (NCTC13786_01645) - 1673646..1675046 (+) 1401 WP_007892964.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  EL147_RS08190 (NCTC13786_01646) - 1675046..1675399 (+) 354 WP_007892969.1 S1 RNA-binding domain-containing protein -
  EL147_RS08195 (NCTC13786_01647) tnpA 1675587..1676042 (+) 456 WP_007896184.1 IS200/IS605 family transposase -
  EL147_RS08200 (NCTC13786_01648) cysK 1676108..1677073 (-) 966 WP_007891534.1 cysteine synthase A -
  EL147_RS08205 (NCTC13786_01649) - 1677186..1677815 (-) 630 WP_007891527.1 YigZ family protein -
  EL147_RS08210 (NCTC13786_01650) comFA/cflA 1677871..1679172 (+) 1302 WP_007891532.1 DEAD/DEAH box helicase Machinery gene
  EL147_RS08215 (NCTC13786_01651) - 1679162..1679842 (+) 681 WP_007891533.1 ComF family protein -
  EL147_RS08220 (NCTC13786_01652) hpf 1679917..1680465 (+) 549 WP_007891529.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49246.13 Da        Isoelectric Point: 9.7308

>NTDB_id=1122026 EL147_RS08210 WP_007891532.1 1677871..1679172(+) (comFA/cflA) [Streptococcus pseudoporcinus strain NCTC13786]
MEDLENLYGRIFTAKQISDEAQKVARTKDSFEGDGKAYYCGRCLSKAGKDHLLPNGHYYCRQCLVFGRVQSHDKLYYFPA
PPFLKGNYLKWKGSLTPYQESISKQLVENYYLGKWSLVYAVTGAGKTEMIYSIIKEVVNSGKWVALVSPRVDVCIEVYKR
LSRDFSCQTILMHAGSESYHRAPLVIATTHQLLKFYKAFHLIIIDEVDSFPFVDNPLLNRAVMSALKEEGQLIYLTATST
KFLESEVTKGKLIKLTLPRRFHNNPLIVPKFQLILNFQNYLDKGKLPTKLYSAIQKQTSLPYPLLIFYPVIEKGQMFYDS
LVKSFPNHQIGYVSSQTAKRKELIEDFRQGSLSILVTTTILERGVTFPGVDVFVVLAHHHLFTSSSLIQIAGRVGRSIDR
PNGKVIFFHEGVSSAMYKARKEIIALNKEAYGS

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=1122026 EL147_RS08210 WP_007891532.1 1677871..1679172(+) (comFA/cflA) [Streptococcus pseudoporcinus strain NCTC13786]
ATGGAAGATTTAGAGAATTTATATGGTCGCATATTCACAGCTAAGCAAATTAGTGACGAAGCACAAAAGGTCGCAAGAAC
GAAAGATAGTTTTGAGGGTGATGGTAAGGCTTATTATTGCGGACGTTGTCTCAGTAAAGCTGGTAAAGATCACTTACTGC
CTAATGGGCATTATTATTGTAGACAATGTTTAGTTTTTGGACGAGTGCAAAGTCATGATAAACTCTATTATTTTCCTGCT
CCGCCATTTTTAAAAGGAAATTATCTCAAGTGGAAAGGAAGCTTAACTCCTTATCAAGAGAGTATTTCTAAACAATTAGT
TGAAAATTATTATTTAGGAAAATGGAGTCTGGTTTATGCTGTTACAGGGGCAGGTAAAACGGAGATGATTTATAGCATTA
TCAAAGAAGTAGTCAATAGTGGGAAATGGGTTGCTTTAGTCAGTCCGAGAGTTGATGTCTGTATTGAAGTCTATAAACGC
TTAAGCCGTGATTTCTCATGTCAGACTATTTTGATGCATGCGGGGTCAGAAAGTTATCATAGAGCTCCGCTTGTTATTGC
CACAACCCACCAGTTATTGAAGTTTTATAAGGCATTTCATTTGATTATTATTGATGAGGTTGATTCTTTTCCCTTTGTTG
ATAATCCCCTCCTAAATCGTGCTGTTATGTCAGCATTGAAAGAGGAAGGTCAGTTAATCTATTTGACAGCCACATCCACG
AAGTTTTTAGAAAGTGAGGTGACCAAAGGGAAACTGATCAAATTAACACTTCCAAGACGATTTCATAATAATCCATTAAT
AGTGCCTAAATTTCAATTGATTTTAAACTTTCAAAACTATTTAGATAAAGGTAAACTACCTACCAAACTATACTCTGCCA
TCCAAAAGCAGACTTCTTTGCCATATCCTCTTTTGATTTTTTATCCTGTCATAGAGAAAGGTCAAATGTTCTATGATAGT
TTAGTAAAATCATTTCCTAATCACCAAATAGGCTATGTAAGTAGTCAAACGGCTAAACGTAAAGAATTGATTGAGGACTT
TCGTCAAGGGAGTCTGTCAATTCTTGTTACGACTACTATACTAGAAAGAGGTGTTACTTTTCCTGGGGTAGATGTCTTTG
TTGTATTAGCGCATCATCACCTTTTTACGTCTTCAAGTCTCATTCAAATAGCAGGGCGTGTTGGGAGATCCATTGATAGA
CCAAATGGTAAGGTAATATTTTTCCATGAGGGAGTGAGCTCTGCTATGTATAAAGCTAGAAAAGAAATAATAGCTTTAAA
TAAGGAAGCTTATGGATCATAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(290-396)

DEAD/DEAH box helicase (DEAD)

(101-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G5K819

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae D39

54.028

97.46

0.527
  comFA/cflA Streptococcus pneumoniae R6

54.028

97.46

0.527
  comFA/cflA Streptococcus pneumoniae TIGR4

54.028

97.46

0.527
  comFA/cflA Streptococcus pneumoniae Rx1

54.028

97.46

0.527
  comFA/cflA Streptococcus mitis SK321

52.706

98.152

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

52.471

98.152

0.515
  comFA Lactococcus lactis subsp. cremoris KW2

47.179

90.069

0.425
  comFA Latilactobacillus sakei subsp. sakei 23K

37.762

99.076

0.374

Multiple sequence alignment