Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   EL144_RS04855 Genome accession   NZ_LR134327
Coordinates   1004799..1005353 (-) Length   184 a.a.
NCBI ID   WP_005703462.1    Uniprot ID   A0A3S4RHG1
Organism   Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 999799..1010353
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL144_RS04830 (NCTC5906_00975) - 999961..1000413 (-) 453 WP_005701641.1 isoprenylcysteine carboxylmethyltransferase family protein -
  EL144_RS04835 (NCTC5906_00976) - 1000469..1001083 (-) 615 WP_005701643.1 YfgM family protein -
  EL144_RS04840 (NCTC5906_00977) hisS 1001094..1002371 (-) 1278 WP_005703457.1 histidine--tRNA ligase -
  EL144_RS04845 (NCTC5906_00978) ispG 1002396..1003499 (-) 1104 WP_005703458.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EL144_RS04850 (NCTC5906_00979) - 1003510..1004580 (-) 1071 WP_032994995.1 RodZ family helix-turn-helix domain-containing protein -
  EL144_RS04855 (NCTC5906_00980) pilF/pilF2 1004799..1005353 (-) 555 WP_005703462.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL144_RS04860 (NCTC5906_00981) - 1005375..1006529 (-) 1155 WP_005703463.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL144_RS04865 (NCTC5906_00982) ansB 1006827..1007876 (+) 1050 WP_005701650.1 L-asparaginase 2 -
  EL144_RS04870 (NCTC5906_00983) - 1008041..1009366 (+) 1326 WP_111297438.1 DUF4123 domain-containing protein -

Sequence

Protein


Download         Length: 184 a.a.        Molecular weight: 21026.96 Da        Isoelectric Point: 7.2833

>NTDB_id=1121598 EL144_RS04855 WP_005703462.1 1004799..1005353(-) (pilF/pilF2) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
MVNIQFIFYRFVPFIFPFLFSACVVHSDAPDFDKQAAAKARVELALGYLQQQDGSQAKLNLDKALSYAPKYYLVHAALAY
FYQQQGDVEHAKQSYLTAIKLDDKQGDVFNNFGAFLCSQGEYQAAYEQFKQALKSLNYYHQADTYENLALCALSAKEQKI
YQENLAALEKIEPVRAKKLTQFVK

Nucleotide


Download         Length: 555 bp        

>NTDB_id=1121598 EL144_RS04855 WP_005703462.1 1004799..1005353(-) (pilF/pilF2) [Aggregatibacter aphrophilus ATCC 33389 strain NCTC 5906]
ATGGTCAATATTCAGTTTATTTTTTACCGTTTTGTACCATTTATTTTTCCTTTTCTGTTTTCTGCCTGTGTTGTCCATTC
CGATGCTCCGGATTTTGATAAACAAGCGGCGGCAAAAGCCCGTGTGGAGTTGGCATTGGGCTATTTACAGCAGCAAGATG
GCAGCCAGGCCAAGTTGAATTTGGATAAAGCCCTTTCTTATGCCCCAAAATATTATTTGGTACATGCCGCGTTAGCCTAT
TTTTATCAGCAACAAGGGGATGTTGAACACGCCAAACAATCGTATTTAACGGCGATTAAACTGGATGATAAACAAGGGGA
TGTATTCAATAATTTCGGTGCGTTTCTTTGCTCACAAGGGGAATATCAGGCCGCGTATGAACAATTTAAACAGGCATTAA
AAAGCCTGAATTATTATCATCAAGCTGACACCTACGAAAACCTTGCACTGTGTGCGTTATCGGCAAAAGAGCAGAAAATT
TATCAAGAGAACCTTGCTGCTCTTGAAAAGATTGAACCGGTACGAGCCAAGAAATTAACTCAGTTCGTCAAATAG

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(75-104)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3S4RHG1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

57.831

90.217

0.522
  pilF Glaesserella parasuis strain SC1401

42.703

100

0.429

Multiple sequence alignment