Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL133_RS02345 Genome accession   NZ_LR134323
Coordinates   463142..464443 (+) Length   433 a.a.
NCBI ID   WP_006738633.1    Uniprot ID   G5KCS1
Organism   Streptococcus urinalis strain NCTC13766     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458142..469443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL133_RS02320 (NCTC13766_00466) - 458962..459603 (+) 642 WP_006739571.1 response regulator transcription factor -
  EL133_RS02325 (NCTC13766_00467) - 459640..461040 (+) 1401 WP_006739756.1 Cof-type HAD-IIB family hydrolase -
  EL133_RS02330 (NCTC13766_00468) - 461040..461402 (+) 363 WP_006739454.1 S1 RNA-binding domain-containing protein -
  EL133_RS02335 (NCTC13766_00469) cysK 461426..462343 (-) 918 WP_006738535.1 cysteine synthase A -
  EL133_RS02340 (NCTC13766_00470) - 462435..463085 (-) 651 WP_006740367.1 YigZ family protein -
  EL133_RS02345 (NCTC13766_00471) comFA/cflA 463142..464443 (+) 1302 WP_006738633.1 DEAD/DEAH box helicase Machinery gene
  EL133_RS02350 (NCTC13766_00472) - 464440..465063 (+) 624 WP_006740323.1 ComF family protein -
  EL133_RS02355 (NCTC13766_00473) hpf 465184..465732 (+) 549 WP_006740469.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49855.51 Da        Isoelectric Point: 9.7046

>NTDB_id=1121521 EL133_RS02345 WP_006738633.1 463142..464443(+) (comFA/cflA) [Streptococcus urinalis strain NCTC13766]
MEELKNFYGRIILKQDVPMQYLSFSKEIPSFILKQNQYYCQRCNSIPEDNHTLPDGQLYCRECLIFGRNSQNIPLVQFDC
PKGIGSDYLVWIGLLTPFQQKISDSLTKLIFEKKKILVHAVTGAGKTEMIYQVVNEFLKIGKWVAIASPRVDVCLELHSR
LSKDFSCGITLLHAQSKGYQWNPIIIMTTHQLLKFRNAFDLLIIDEVDAFPYVGNDVLHFATNHAIKEEGCHIFLTATST
KKLEREIQKGRLYKLTLPRRFHGNPLVVPKLFCLNNFKNQLEKGRLPKKLIIDIENQRKTNFPLLCFVPTIVLGEQLKKV
LEKLFEKEAIAFVCSQTINRHEIIELFRLKKYSILITSTILERGVTFPGVDVFVLEAHHYLFTKSSLIQIAGRVGRSIER
PTGKLNFYHNGKTLALVNAIKDIKQMNRKGEQI

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=1121521 EL133_RS02345 WP_006738633.1 463142..464443(+) (comFA/cflA) [Streptococcus urinalis strain NCTC13766]
ATGGAAGAATTAAAGAATTTTTATGGAAGAATTATTTTAAAGCAAGATGTACCAATGCAATATCTATCGTTTTCTAAAGA
AATACCGTCGTTTATACTCAAACAAAATCAGTACTATTGTCAAAGGTGTAATAGTATTCCAGAAGATAACCATACCCTTC
CTGATGGACAACTCTACTGTCGTGAGTGTCTGATTTTTGGGAGAAATAGTCAAAACATTCCTTTAGTTCAATTTGATTGT
CCAAAAGGAATTGGTTCAGACTACCTAGTCTGGATAGGACTTTTAACACCGTTTCAGCAAAAAATTTCGGATAGTCTAAC
AAAATTAATTTTTGAAAAGAAAAAGATATTAGTACATGCTGTAACCGGAGCTGGAAAAACAGAAATGATTTACCAAGTAG
TAAATGAATTTTTAAAAATTGGGAAATGGGTAGCTATTGCAAGTCCTCGAGTTGATGTCTGTTTGGAACTTCATTCAAGG
TTATCAAAAGATTTCTCTTGTGGAATTACCTTATTGCATGCTCAGTCTAAAGGCTATCAATGGAACCCCATTATTATTAT
GACAACTCATCAATTGTTAAAATTTAGAAATGCATTTGATTTATTAATCATTGATGAAGTAGATGCTTTTCCATATGTTG
GTAATGATGTCTTACATTTTGCGACTAACCATGCTATCAAAGAAGAGGGATGTCATATTTTTTTGACGGCAACTTCAACA
AAAAAATTAGAAAGAGAAATTCAAAAAGGTAGATTATACAAATTGACTTTACCAAGAAGATTTCACGGTAATCCATTAGT
GGTACCTAAATTATTTTGCTTAAATAATTTTAAAAATCAATTAGAAAAAGGAAGACTTCCCAAAAAGTTAATTATTGATA
TTGAGAACCAACGAAAAACAAATTTTCCATTATTATGTTTTGTGCCAACAATTGTATTAGGTGAGCAATTAAAAAAAGTA
CTTGAAAAATTATTTGAAAAAGAGGCAATTGCATTTGTATGTAGTCAAACTATTAACAGACATGAGATAATCGAATTATT
TCGTCTAAAGAAATATTCGATATTGATAACAAGCACCATACTAGAAAGAGGAGTTACTTTTCCAGGCGTTGATGTTTTTG
TTTTGGAAGCACACCATTATTTATTTACAAAAAGTTCTCTAATTCAGATTGCAGGAAGAGTTGGTAGATCCATTGAAAGA
CCGACAGGAAAGCTAAATTTTTATCATAATGGTAAAACGCTAGCTTTAGTAAATGCAATAAAAGATATTAAACAAATGAA
TAGAAAAGGCGAGCAGATATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(296-396)

DEAD/DEAH box helicase (DEAD)

(105-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G5KCS1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

52.103

98.845

0.515
  comFA/cflA Streptococcus pneumoniae TIGR4

51.402

98.845

0.508
  comFA/cflA Streptococcus pneumoniae Rx1

51.402

98.845

0.508
  comFA/cflA Streptococcus pneumoniae D39

51.402

98.845

0.508
  comFA/cflA Streptococcus pneumoniae R6

51.402

98.845

0.508
  comFA/cflA Streptococcus mitis SK321

51.529

98.152

0.506
  comFA Lactococcus lactis subsp. cremoris KW2

51.128

92.148

0.471
  comFA Latilactobacillus sakei subsp. sakei 23K

38.756

96.536

0.374

Multiple sequence alignment