Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   EL295_RS32465 Genome accession   NZ_LR134308
Coordinates   6703399..6704991 (-) Length   530 a.a.
NCBI ID   WP_003110428.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NCTC11445     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 6698399..6709991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL295_RS32440 (NCTC11445_06359) pilV 6699103..6699660 (-) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  EL295_RS32445 (NCTC11445_06360) - 6699651..6700157 (-) 507 WP_003102598.1 GspH/FimT family pseudopilin -
  EL295_RS32450 (NCTC11445_06361) - 6700263..6700772 (-) 510 WP_003102595.1 GspH/FimT family pseudopilin -
  EL295_RS32455 (NCTC11445_06362) thiO 6700907..6702001 (+) 1095 WP_012614545.1 glycine oxidase ThiO -
  EL295_RS32460 (NCTC11445_06363) pilR 6702047..6703384 (-) 1338 WP_003110427.1 two-component system response regulator PilR Regulator
  EL295_RS32465 (NCTC11445_06364) pilS 6703399..6704991 (-) 1593 WP_003110428.1 two-component system sensor histidine kinase PilS Regulator
  EL295_RS32470 (NCTC11445_06365) - 6704981..6705226 (-) 246 WP_009316269.1 PP0621 family protein -
  EL295_RS32475 (NCTC11445_06366) - 6705383..6706408 (-) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  EL295_RS32480 (NCTC11445_06367) rluD 6706554..6707516 (+) 963 WP_033973880.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  EL295_RS32485 (NCTC11445_06368) pgeF 6707513..6708241 (+) 729 WP_003102588.1 peptidoglycan editing factor PgeF -

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 58986.14 Da        Isoelectric Point: 6.5650

>NTDB_id=1120958 EL295_RS32465 WP_003110428.1 6703399..6704991(-) (pilS) [Pseudomonas aeruginosa strain NCTC11445]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGAGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVPADKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=1120958 EL295_RS32465 WP_003110428.1 6703399..6704991(-) (pilS) [Pseudomonas aeruginosa strain NCTC11445]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCACCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTACCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCGGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGTCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCTCTGGTGCGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAACTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTGCAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCGACGCTCAAGGTGGT
GCCGGACGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAGGAGTCAGAGGAACTGGATGCCCCGGACCGACG
GCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATATCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAGCTCGGTGCCGGCGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGGCGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.811

100

0.998

Multiple sequence alignment