Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EL119_RS06515 Genome accession   NZ_LR134305
Coordinates   1271278..1272252 (-) Length   324 a.a.
NCBI ID   WP_000697220.1    Uniprot ID   W8U5L8
Organism   Staphylococcus aureus strain NCTC1803     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1266278..1277252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL119_RS06475 (NCTC1803_01227) gcvT 1266780..1267871 (-) 1092 WP_000093343.1 glycine cleavage system aminomethyltransferase GcvT -
  EL119_RS06480 - 1268029..1268553 (-) 525 WP_001015120.1 shikimate kinase -
  EL119_RS06485 - 1268543..1268689 (-) 147 WP_001789879.1 hypothetical protein -
  EL119_RS06490 (NCTC1803_01229) comGF 1268786..1269283 (-) 498 WP_078061106.1 competence type IV pilus minor pilin ComGF Machinery gene
  EL119_RS06495 (NCTC1803_01230) comGE 1269201..1269500 (-) 300 WP_000844409.1 hypothetical protein Machinery gene
  EL119_RS06500 (NCTC1803_01231) comGD 1269487..1269933 (-) 447 WP_001796473.1 competence type IV pilus minor pilin ComGD Machinery gene
  EL119_RS06505 (NCTC1803_01232) comGC 1269911..1270222 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EL119_RS06510 (NCTC1803_01233) comGB 1270236..1271306 (-) 1071 WP_000776418.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL119_RS06515 (NCTC1803_01234) comGA 1271278..1272252 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EL119_RS06520 (NCTC1803_01235) - 1272304..1272927 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EL119_RS06525 (NCTC1803_01236) - 1272924..1273253 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EL119_RS06530 (NCTC1803_01237) - 1273253..1274239 (-) 987 WP_000161315.1 ROK family glucokinase -
  EL119_RS06535 - 1274236..1274439 (-) 204 WP_000087561.1 YqgQ family protein -
  EL119_RS06540 (NCTC1803_01238) - 1274420..1275883 (-) 1464 WP_001017867.1 rhomboid family protein -
  EL119_RS06545 (NCTC1803_01239) - 1275895..1276434 (-) 540 WP_000164552.1 5-formyltetrahydrofolate cyclo-ligase -
  EL119_RS06550 (NCTC1803_01240) rpmG 1276643..1276792 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36897.96 Da        Isoelectric Point: 8.8516

>NTDB_id=1120821 EL119_RS06515 WP_000697220.1 1271278..1272252(-) (comGA) [Staphylococcus aureus strain NCTC1803]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1120821 EL119_RS06515 WP_000697220.1 1271278..1272252(-) (comGA) [Staphylococcus aureus strain NCTC1803]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCATTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTAATCAGAATTGTACCTCA
ATTTTTTCAACAACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCTACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAAGATCCTGTAGAGATGCAAATTCCTGGTATTGTCCAAATTAATGTGAATGATAAAGCTGGCATTAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
GATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W8U5L8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

100

100

1
  comGA Staphylococcus aureus N315

100

100

1

Multiple sequence alignment