Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   EL104_RS00805 Genome accession   NZ_LR134298
Coordinates   161267..161812 (+) Length   181 a.a.
NCBI ID   WP_074866039.1    Uniprot ID   -
Organism   Pasteurella multocida subsp. gallicida strain NCTC10204     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 156267..166812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL104_RS00790 (NCTC10204_00161) - 156448..157701 (+) 1254 WP_059245744.1 AI-2E family transporter -
  EL104_RS00795 (NCTC10204_00162) - 157818..159863 (+) 2046 WP_074866033.1 tetratricopeptide repeat protein -
  EL104_RS00800 (NCTC10204_00163) - 160043..161227 (+) 1185 WP_074866036.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  EL104_RS00805 (NCTC10204_00164) pilF/pilF2 161267..161812 (+) 546 WP_074866039.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  EL104_RS00810 (NCTC10204_00165) - 161935..162891 (+) 957 WP_074866042.1 helix-turn-helix domain-containing protein -
  EL104_RS00815 (NCTC10204_00166) ispG 162918..164021 (+) 1104 WP_005719572.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  EL104_RS00820 (NCTC10204_00167) hisS 164051..165322 (+) 1272 WP_005725135.1 histidine--tRNA ligase -
  EL104_RS00825 (NCTC10204_00168) - 165341..165952 (+) 612 WP_005725137.1 YfgM family protein -
  EL104_RS00830 (NCTC10204_00169) - 166165..166602 (+) 438 WP_010907449.1 methyltransferase family protein -

Sequence

Protein


Download         Length: 181 a.a.        Molecular weight: 20879.47 Da        Isoelectric Point: 7.8913

>NTDB_id=1120683 EL104_RS00805 WP_074866039.1 161267..161812(+) (pilF/pilF2) [Pasteurella multocida subsp. gallicida strain NCTC10204]
MTRVFQIWIASIFSLFLAACVSQEGNKTFDSHEAAKARIELGLGYLSQHDFARAKQNFDKALSHAPNYYLSHAVLAYIYQ
KQGHLDLARHAYEKAIKLDKNQGDVLNNYGAFLCQQGEFEQAYKQFSQALNAPHYYQHADTYENLVWCASFAKDAVRYQQ
NLQHLTQFDPNRAKKFPPHIE

Nucleotide


Download         Length: 546 bp        

>NTDB_id=1120683 EL104_RS00805 WP_074866039.1 161267..161812(+) (pilF/pilF2) [Pasteurella multocida subsp. gallicida strain NCTC10204]
ATGACGCGCGTTTTTCAAATATGGATAGCAAGCATTTTTTCTCTTTTTCTTGCTGCCTGTGTGAGTCAAGAAGGTAACAA
AACGTTTGATTCACATGAGGCGGCAAAAGCCAGAATTGAATTAGGTTTAGGCTATTTATCTCAGCATGATTTTGCGCGAG
CAAAGCAAAATTTTGATAAAGCTTTATCCCATGCCCCAAATTACTATTTATCTCATGCGGTCTTAGCGTATATCTACCAA
AAACAAGGTCATCTTGATTTAGCCCGTCACGCTTATGAAAAGGCGATAAAATTAGATAAAAATCAAGGCGATGTATTAAA
TAATTATGGTGCCTTTTTATGCCAACAAGGTGAGTTTGAACAAGCTTACAAACAATTTTCTCAAGCATTAAATGCCCCAC
ATTATTATCAACACGCTGATACTTATGAAAATTTAGTTTGGTGTGCGTCTTTTGCCAAAGACGCTGTCCGTTATCAACAA
AATTTGCAACATTTAACGCAGTTTGATCCCAATAGAGCAAAAAAATTTCCGCCACATATTGAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

49.432

97.238

0.481
  pilF Glaesserella parasuis strain SC1401

43.85

100

0.453

Multiple sequence alignment