Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL070_RS03045 Genome accession   NZ_LR134284
Coordinates   571356..572681 (+) Length   441 a.a.
NCBI ID   WP_111705720.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8232     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 566356..577681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL070_RS03020 (NCTC8232_00600) - 567126..567767 (+) 642 WP_002983671.1 response regulator transcription factor -
  EL070_RS03025 (NCTC8232_00601) - 567804..569204 (+) 1401 WP_111705724.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  EL070_RS03030 (NCTC8232_00602) - 569204..569581 (+) 378 WP_111705723.1 S1 RNA-binding domain-containing protein -
  EL070_RS03035 (NCTC8232_00603) cysK 569599..570540 (-) 942 WP_111705722.1 cysteine synthase A -
  EL070_RS03040 (NCTC8232_00604) - 570668..571300 (-) 633 WP_111705721.1 YigZ family protein -
  EL070_RS03045 (NCTC8232_00605) comFA/cflA 571356..572681 (+) 1326 WP_111705720.1 DEAD/DEAH box helicase Machinery gene
  EL070_RS03050 (NCTC8232_00606) - 572653..573318 (+) 666 WP_002993195.1 ComF family protein -
  EL070_RS03055 (NCTC8232_00607) hpf 573398..573946 (+) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49870.36 Da        Isoelectric Point: 10.0084

>NTDB_id=1120101 EL070_RS03045 WP_111705720.1 571356..572681(+) (comFA/cflA) [Streptococcus pyogenes strain NCTC8232]
MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESFPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRLLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTIIMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1120101 EL070_RS03045 WP_111705720.1 571356..572681(+) (comFA/cflA) [Streptococcus pyogenes strain NCTC8232]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAGGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTTTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGTTATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTATAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGCGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae D39

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae R6

54.177

95.011

0.515
  comFA/cflA Streptococcus pneumoniae TIGR4

54.177

95.011

0.515
  comFA/cflA Streptococcus mitis NCTC 12261

52.844

95.692

0.506
  comFA/cflA Streptococcus mitis SK321

51.896

95.692

0.497
  comFA Lactococcus lactis subsp. cremoris KW2

47.118

90.476

0.426
  comFA Latilactobacillus sakei subsp. sakei 23K

40.099

91.61

0.367

Multiple sequence alignment