Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EL020_RS08435 Genome accession   NZ_LR134203
Coordinates   1656830..1658128 (-) Length   432 a.a.
NCBI ID   WP_020916319.1    Uniprot ID   -
Organism   Streptococcus lutetiensis strain NCTC11436     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1651830..1663128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL020_RS08425 (NCTC11436_01802) hpf 1655543..1656091 (-) 549 WP_058813685.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EL020_RS08430 (NCTC11436_01803) - 1656168..1656830 (-) 663 WP_020916320.1 ComF family protein -
  EL020_RS08435 (NCTC11436_01804) comFA/cflA 1656830..1658128 (-) 1299 WP_020916319.1 DEAD/DEAH box helicase Machinery gene
  EL020_RS08440 (NCTC11436_01805) - 1658183..1658809 (+) 627 WP_020916318.1 YigZ family protein -
  EL020_RS08445 (NCTC11436_01806) cysK 1658909..1659838 (+) 930 WP_020916317.1 cysteine synthase A -
  EL020_RS08450 (NCTC11436_01807) - 1659937..1660293 (-) 357 WP_020916316.1 S1 RNA-binding domain-containing protein -
  EL020_RS10010 (NCTC11436_01808) - 1660293..1661693 (-) 1401 WP_081048448.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  EL020_RS08460 (NCTC11436_01809) - 1661817..1662461 (-) 645 WP_020916314.1 response regulator -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 48911.30 Da        Isoelectric Point: 10.1284

>NTDB_id=1118981 EL020_RS08435 WP_020916319.1 1656830..1658128(-) (comFA/cflA) [Streptococcus lutetiensis strain NCTC11436]
MTELNDYYGRIFTGSQLTDDLKKKAKALPSVKKEKGQMICNRCGSVMAANQMLQTGRIYCRECLVFGRNTSDSNLYYFPQ
KPFTKTQSLKWQGQLTSYQGEVSKGMLKGILEKQDLLIHAVTGAGKTEMIYQSLAKVIDNGGSVCLASPRIDVCLELYKR
LSRDFSCPITLLHGDSEPYKRSPLVIITTHQLLKFYRAFDLLIIDEVDAFPFVDNKMLYHAVNQSLKDDGVKLFLTATST
DELDKKVKKGELTKLHLARRFHANPLVVPKIVWLSKVLISMKKKKLPPKLISLLKKQRQSQFPLLIFFPNIEQGREFTSI
LQTTFPDERIGFVSSKTENRQELVEEFRQGKLTILVTTTILERGVTFPCVDVFVLLANHKLYSKSALVQISGRVGRAAER
PTGELLFLHDGVTKAMKKAISEIKEMNKKGGF

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=1118981 EL020_RS08435 WP_020916319.1 1656830..1658128(-) (comFA/cflA) [Streptococcus lutetiensis strain NCTC11436]
ATGACAGAGTTGAATGATTACTATGGAAGAATTTTTACAGGAAGTCAATTAACAGATGACTTGAAAAAGAAAGCAAAAGC
CCTTCCTTCGGTTAAAAAGGAAAAAGGGCAAATGATTTGCAATCGCTGTGGAAGTGTAATGGCAGCAAATCAAATGTTGC
AAACTGGTCGCATTTATTGTAGAGAGTGTTTAGTTTTTGGACGAAATACTAGTGATTCTAACCTTTATTATTTTCCTCAG
AAGCCTTTTACAAAGACTCAGTCGTTAAAGTGGCAAGGGCAATTGACTTCTTATCAAGGTGAAGTTTCTAAAGGCATGCT
TAAAGGCATTTTAGAAAAACAAGATTTATTGATTCACGCTGTGACTGGTGCTGGTAAAACTGAAATGATTTATCAATCTT
TAGCCAAAGTTATTGATAATGGAGGAAGTGTCTGCTTAGCAAGCCCAAGGATTGATGTTTGTTTAGAACTTTATAAACGG
TTATCCAGAGATTTTTCTTGCCCTATTACGCTTTTACATGGTGATTCTGAGCCGTATAAACGCTCTCCCTTGGTAATTAT
AACGACCCATCAGCTTTTAAAATTTTACCGAGCTTTTGATTTATTAATCATTGACGAAGTTGATGCCTTTCCCTTTGTTG
ATAATAAAATGCTTTATCACGCGGTCAACCAATCCTTAAAAGATGATGGCGTTAAACTCTTTTTAACAGCGACTTCGACT
GATGAACTTGATAAAAAGGTGAAAAAAGGCGAGCTGACAAAGCTGCATTTGGCAAGACGTTTTCATGCCAATCCTTTGGT
AGTCCCTAAAATAGTTTGGTTAAGTAAAGTGCTAATCTCAATGAAAAAGAAAAAACTTCCTCCTAAACTAATCTCTCTTT
TAAAAAAGCAAAGGCAAAGTCAATTTCCGCTTCTTATCTTTTTTCCGAATATTGAACAAGGTAGAGAATTTACAAGTATC
TTACAAACCACTTTTCCAGATGAAAGAATAGGATTTGTATCAAGTAAAACTGAAAATCGTCAGGAATTGGTAGAAGAATT
TAGACAAGGAAAATTAACAATATTGGTGACAACAACCATCTTAGAACGCGGAGTCACTTTTCCTTGTGTTGATGTTTTTG
TTCTACTTGCAAATCATAAATTGTACAGCAAAAGTGCGCTTGTACAGATATCAGGACGAGTTGGACGAGCAGCTGAAAGA
CCAACAGGAGAATTGCTATTTTTACATGATGGAGTGACAAAAGCAATGAAAAAAGCTATTTCAGAAATAAAAGAGATGAA
TAAAAAAGGAGGATTCTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-397)

DEAD/DEAH box helicase (DEAD)

(107-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

61.124

98.843

0.604
  comFA/cflA Streptococcus pneumoniae D39

61.124

98.843

0.604
  comFA/cflA Streptococcus pneumoniae R6

61.124

98.843

0.604
  comFA/cflA Streptococcus pneumoniae TIGR4

61.124

98.843

0.604
  comFA/cflA Streptococcus mitis NCTC 12261

60.656

98.843

0.6
  comFA/cflA Streptococcus mitis SK321

60.656

98.843

0.6
  comFA Lactococcus lactis subsp. cremoris KW2

50.488

94.907

0.479
  comFA Latilactobacillus sakei subsp. sakei 23K

38.303

100

0.387

Multiple sequence alignment