Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ELZ63_RS01715 Genome accession   NZ_LR134171
Coordinates   338853..339392 (-) Length   179 a.a.
NCBI ID   WP_065245016.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC12699     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333853..344392
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ELZ63_RS01685 (NCTC12699_00335) fdx 334256..334597 (+) 342 WP_005656373.1 ISC system 2Fe-2S type ferredoxin -
  ELZ63_RS01690 (NCTC12699_00336) iscX 334597..334791 (+) 195 WP_005626949.1 Fe-S cluster assembly protein IscX -
  ELZ63_RS01695 (NCTC12699_00337) - 334842..335456 (-) 615 WP_005649148.1 YfgM family protein -
  ELZ63_RS01700 (NCTC12699_00338) hisS 335474..336745 (-) 1272 WP_032823940.1 histidine--tRNA ligase -
  ELZ63_RS01705 (NCTC12699_00339) ispG 336755..337861 (-) 1107 WP_050829089.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  ELZ63_RS01710 (NCTC12699_00340) - 337870..338781 (-) 912 WP_065245017.1 RodZ family helix-turn-helix domain-containing protein -
  ELZ63_RS01715 (NCTC12699_00341) pilF/pilF2 338853..339392 (-) 540 WP_065245016.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ELZ63_RS01720 (NCTC12699_00342) - 339493..340644 (-) 1152 WP_042594886.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ELZ63_RS01725 (NCTC12699_00343) - 340891..341769 (+) 879 WP_065245015.1 serine hydrolase -
  ELZ63_RS01730 (NCTC12699_00344) - 342135..343016 (+) 882 WP_005662662.1 metal ABC transporter substrate-binding protein -
  ELZ63_RS01735 (NCTC12699_00345) - 343016..343936 (+) 921 WP_005662663.1 ATP-binding cassette domain-containing protein -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 20559.17 Da        Isoelectric Point: 8.2626

>NTDB_id=1118731 ELZ63_RS01715 WP_065245016.1 338853..339392(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC12699]
MKTISKQLSAVIFPFIFSACVSQSASSLNHQAAAKARVELALSYLQQNNPQLAKINLDKALQHDKNYYLVHSALAHYYQQ
QGEIENAHREYEIAVNLNHKQGDVHNNFGTFLCSQKKFEQAQQQFELALNSPNYYHQADTFENIVLCAYSAQKMDIYQQT
LEKLRQIDGKRAEKFNSLK

Nucleotide


Download         Length: 540 bp        

>NTDB_id=1118731 ELZ63_RS01715 WP_065245016.1 338853..339392(-) (pilF/pilF2) [Haemophilus influenzae strain NCTC12699]
ATGAAAACAATAAGTAAACAACTAAGTGCGGTTATTTTTCCTTTTATTTTTTCTGCCTGCGTTTCACAATCTGCATCTAG
CTTAAATCATCAAGCTGCCGCTAAAGCGCGTGTGGAACTCGCTTTGAGCTATCTTCAGCAAAATAATCCTCAACTGGCTA
AAATCAATTTAGATAAAGCACTTCAACACGATAAAAATTACTATCTCGTGCATTCAGCACTTGCACATTATTATCAACAA
CAAGGGGAAATAGAGAATGCACATCGTGAATATGAAATAGCCGTAAACCTTAATCATAAACAAGGCGATGTGCATAATAA
TTTTGGTACGTTTCTATGTAGTCAAAAGAAATTTGAACAAGCCCAGCAACAATTTGAATTAGCACTTAATTCGCCAAATT
ATTATCATCAAGCAGATACATTTGAAAATATCGTGCTTTGTGCTTATTCCGCGCAAAAAATGGATATTTATCAGCAAACA
TTAGAAAAATTACGTCAAATCGATGGAAAACGTGCGGAAAAATTCAATAGCCTAAAATAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

98.324

100

0.983
  pilF Glaesserella parasuis strain SC1401

44.693

100

0.447

Multiple sequence alignment