Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EL050_RS13645 Genome accession   NZ_LR134165
Coordinates   2720144..2721208 (-) Length   354 a.a.
NCBI ID   WP_025811157.1    Uniprot ID   -
Organism   Bacillus paralicheniformis strain NCTC8721     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2715144..2726208
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL050_RS13600 (NCTC8721_02794) tapA 2715637..2716365 (-) 729 WP_020452169.1 amyloid fiber anchoring/assembly protein TapA -
  EL050_RS13605 (NCTC8721_02795) - 2716643..2716963 (+) 321 WP_023855188.1 YqzG/YhdC family protein -
  EL050_RS13610 (NCTC8721_02796) - 2716993..2717175 (-) 183 WP_025811164.1 YqzE family protein -
  EL050_RS13615 (NCTC8721_02797) comGG 2717265..2717630 (-) 366 WP_025811163.1 competence type IV pilus minor pilin ComGG -
  EL050_RS13620 (NCTC8721_02798) comGF 2717642..2718124 (-) 483 WP_238550077.1 competence type IV pilus minor pilin ComGF -
  EL050_RS13625 comGE 2718039..2718386 (-) 348 WP_025811161.1 competence type IV pilus minor pilin ComGE -
  EL050_RS13630 (NCTC8721_02799) comGD 2718370..2718807 (-) 438 WP_025811160.1 competence type IV pilus minor pilin ComGD -
  EL050_RS13635 (NCTC8721_02800) comGC 2718813..2719106 (-) 294 WP_025811159.1 competence type IV pilus major pilin ComGC Machinery gene
  EL050_RS13640 (NCTC8721_02801) comGB 2719120..2720154 (-) 1035 WP_025811158.1 competence type IV pilus assembly protein ComGB Machinery gene
  EL050_RS13645 (NCTC8721_02802) comGA 2720144..2721208 (-) 1065 WP_025811157.1 competence type IV pilus ATPase ComGA Machinery gene
  EL050_RS13650 (NCTC8721_02803) - 2721366..2722205 (-) 840 WP_020452179.1 STAS domain-containing protein -
  EL050_RS13660 (NCTC8721_02805) - 2722447..2722824 (-) 378 WP_003183482.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  EL050_RS13665 (NCTC8721_02806) - 2723035..2723280 (+) 246 WP_020452180.1 DUF2626 domain-containing protein -
  EL050_RS13670 (NCTC8721_02807) - 2723316..2723954 (-) 639 WP_025811156.1 MBL fold metallo-hydrolase -
  EL050_RS13675 (NCTC8721_02808) - 2724110..2724283 (+) 174 WP_003183486.1 DUF2759 domain-containing protein -
  EL050_RS13680 (NCTC8721_02809) - 2724344..2724658 (-) 315 WP_020452182.1 MTH1187 family thiamine-binding protein -
  EL050_RS13685 (NCTC8721_02810) - 2724674..2725783 (-) 1110 WP_020452183.1 hypothetical protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39797.28 Da        Isoelectric Point: 9.1063

>NTDB_id=1118604 EL050_RS13645 WP_025811157.1 2720144..2721208(-) (comGA) [Bacillus paralicheniformis strain NCTC8721]
MYTIESLSGRLIDEAYRMKASDIHIVPGEKEAVVRFRIDDELFQKDRLTRNECSRLISHFKFLSSMDIGERRLPQSGALT
LYINRQPVHLRMSTLPTIHDESLVIRLLPKMSSKPLTKLSLFPSATFKLLSFLKHSHGLILFTGPTGSGKTTTLYSLIEY
AKRHFKRNIITLEDPVESRSENILQVQVNEKAGMTYSAGLKAVLRHDPDMIILGEIRDAETAQIAVRAALTGHLVLSSMH
AKNAKGAIYRLLEFGVDMTEIEQTLVAISAQRLVGLVCPFCGDSCSLYCRLSRPVRRASVFELLYGKSLNLCIEEAKGRC
GDIKTETLKTLIQKGIALGYLPSNTYERWIGHED

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1118604 EL050_RS13645 WP_025811157.1 2720144..2721208(-) (comGA) [Bacillus paralicheniformis strain NCTC8721]
TTGTACACGATTGAATCATTAAGCGGAAGATTAATTGACGAGGCATATAGAATGAAGGCGTCAGACATTCATATTGTTCC
CGGTGAAAAGGAGGCGGTGGTCCGCTTCAGGATTGATGATGAACTTTTTCAAAAAGACAGATTAACGAGAAATGAGTGCT
CAAGGCTCATTTCTCACTTTAAATTCCTTTCTTCGATGGATATTGGGGAAAGACGGCTGCCGCAAAGCGGAGCTTTAACT
CTTTATATCAACCGTCAGCCCGTTCATTTAAGAATGTCCACTCTGCCCACCATCCATGATGAAAGCTTGGTCATCAGGCT
TCTTCCCAAGATGAGCAGCAAGCCGCTCACAAAGCTCTCATTGTTTCCGAGTGCAACATTCAAGCTGCTGTCTTTTCTGA
AGCATTCCCACGGATTAATTCTGTTCACCGGTCCGACAGGCTCGGGGAAAACGACAACACTCTATTCTTTAATTGAATAC
GCTAAGCGGCATTTTAAGCGAAATATCATTACCTTGGAAGACCCTGTTGAATCAAGAAGCGAAAACATTTTGCAAGTGCA
AGTAAACGAAAAAGCTGGCATGACTTATTCTGCGGGGTTGAAGGCGGTGCTTCGCCACGATCCGGACATGATCATCCTAG
GGGAAATTCGCGATGCCGAAACAGCCCAAATCGCAGTCAGGGCAGCTTTGACAGGACACCTTGTTTTATCGAGCATGCAT
GCGAAAAATGCCAAAGGCGCAATATACAGGCTGCTCGAGTTCGGCGTTGATATGACAGAAATTGAACAAACACTGGTCGC
AATCAGCGCCCAGCGCCTCGTCGGTCTCGTCTGCCCGTTTTGCGGCGACAGCTGCTCTTTATATTGCAGATTGTCGAGGC
CTGTGAGAAGAGCAAGCGTCTTCGAGCTTTTGTACGGAAAAAGCCTCAATCTCTGCATCGAAGAAGCAAAAGGCAGGTGC
GGTGACATAAAAACAGAAACTTTGAAAACGCTGATCCAGAAGGGAATAGCACTCGGATATCTGCCGTCGAATACTTATGA
ACGCTGGATAGGCCATGAAGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

67.797

100

0.678
  pilB Vibrio cholerae strain A1552

38.671

93.503

0.362

Multiple sequence alignment