Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   EW033_RS08780 Genome accession   NZ_LR130515
Coordinates   1720248..1720934 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus strain BPH2947 isolate BPH2947     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1715248..1725934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW033_RS08760 - 1716157..1716573 (-) 417 Protein_1650 ComEC/Rec2 family competence protein -
  EW033_RS08765 - 1716671..1717843 (+) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  EW033_RS08770 - 1717830..1719690 (-) 1861 Protein_1652 ComEC/Rec2 family competence protein -
  EW033_RS08775 - 1719695..1720156 (-) 462 WP_000439693.1 ComE operon protein 2 -
  EW033_RS08780 comEA 1720248..1720934 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  EW033_RS08785 - 1720974..1721690 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  EW033_RS08790 rsfS 1721693..1722046 (-) 354 WP_001088022.1 ribosome silencing factor -
  EW033_RS08795 yqeK 1722047..1722631 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EW033_RS08800 nadD 1722621..1723190 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  EW033_RS08805 yhbY 1723193..1723483 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  EW033_RS08810 aroE 1723487..1724293 (-) 807 WP_000666761.1 shikimate dehydrogenase -
  EW033_RS08815 yqeH 1724307..1725407 (-) 1101 WP_001280148.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=1116910 EW033_RS08780 WP_001792568.1 1720248..1720934(-) (comEA) [Staphylococcus aureus strain BPH2947 isolate BPH2947]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1116910 EW033_RS08780 WP_001792568.1 1720248..1720934(-) (comEA) [Staphylococcus aureus strain BPH2947 isolate BPH2947]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment