Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   EW033_RS08540 Genome accession   NZ_LR130515
Coordinates   1674040..1675014 (-) Length   324 a.a.
NCBI ID   WP_000697228.1    Uniprot ID   A0A0D3Q868
Organism   Staphylococcus aureus strain BPH2947 isolate BPH2947     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1669040..1680014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW033_RS08500 gcvT 1669541..1670632 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  EW033_RS08505 - 1670791..1671315 (-) 525 WP_001015117.1 shikimate kinase -
  EW033_RS08510 - 1671305..1671451 (-) 147 WP_001789879.1 hypothetical protein -
  EW033_RS08515 comGF 1671548..1672045 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW033_RS08520 comGE 1671963..1672262 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  EW033_RS08525 comGD 1672249..1672695 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  EW033_RS08530 comGC 1672673..1672984 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EW033_RS08535 comGB 1672998..1674068 (-) 1071 WP_000775706.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW033_RS08540 comGA 1674040..1675014 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  EW033_RS08545 - 1675066..1675689 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EW033_RS08550 - 1675686..1676015 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EW033_RS08555 - 1676015..1677001 (-) 987 WP_000161314.1 ROK family glucokinase -
  EW033_RS08560 - 1676998..1677201 (-) 204 WP_000087561.1 YqgQ family protein -
  EW033_RS08565 - 1677182..1678645 (-) 1464 WP_001017867.1 rhomboid family protein -
  EW033_RS08570 - 1678657..1679196 (-) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  EW033_RS08575 rpmG 1679405..1679554 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36865.01 Da        Isoelectric Point: 9.0399

>NTDB_id=1116908 EW033_RS08540 WP_000697228.1 1674040..1675014(-) (comGA) [Staphylococcus aureus strain BPH2947 isolate BPH2947]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=1116908 EW033_RS08540 WP_000697228.1 1674040..1675014(-) (comGA) [Staphylococcus aureus strain BPH2947 isolate BPH2947]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D3Q868

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991

Multiple sequence alignment