Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB   Type   Machinery gene
Locus tag   EW033_RS08535 Genome accession   NZ_LR130515
Coordinates   1672998..1674068 (-) Length   356 a.a.
NCBI ID   WP_000775706.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BPH2947 isolate BPH2947     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1667998..1679068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW033_RS08495 gcvPA 1668175..1669521 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EW033_RS08500 gcvT 1669541..1670632 (-) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  EW033_RS08505 - 1670791..1671315 (-) 525 WP_001015117.1 shikimate kinase -
  EW033_RS08510 - 1671305..1671451 (-) 147 WP_001789879.1 hypothetical protein -
  EW033_RS08515 comGF 1671548..1672045 (-) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW033_RS08520 comGE 1671963..1672262 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  EW033_RS08525 comGD 1672249..1672695 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  EW033_RS08530 comGC 1672673..1672984 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EW033_RS08535 comGB 1672998..1674068 (-) 1071 WP_000775706.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW033_RS08540 comGA 1674040..1675014 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  EW033_RS08545 - 1675066..1675689 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EW033_RS08550 - 1675686..1676015 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EW033_RS08555 - 1676015..1677001 (-) 987 WP_000161314.1 ROK family glucokinase -
  EW033_RS08560 - 1676998..1677201 (-) 204 WP_000087561.1 YqgQ family protein -
  EW033_RS08565 - 1677182..1678645 (-) 1464 WP_001017867.1 rhomboid family protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 41391.84 Da        Isoelectric Point: 10.0540

>NTDB_id=1116907 EW033_RS08535 WP_000775706.1 1672998..1674068(-) (comGB) [Staphylococcus aureus strain BPH2947 isolate BPH2947]
MKLQCINTFKLHSKKRQLSKAQQIDLLSNLCNLLKYGFTLYQSFQFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIG
YSDTIVMQVYLAERFGNIIDVLEETVNYMKVNRKSEQRLLKKLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
SFQKTLSFFITSLPTIIVVMLIIVSMLAIIMKLIYNNLNMLNKINFVMKLPLISGYFQLFKTYFVTNELVLFYKNGITLQ
SIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKPQLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIK
QTQFLQPILFLILGLFIVAIYLVIMLPMFQMMQSIK

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1116907 EW033_RS08535 WP_000775706.1 1672998..1674068(-) (comGB) [Staphylococcus aureus strain BPH2947 isolate BPH2947]
GTGAAACTACAATGCATAAATACATTTAAACTACATTCTAAGAAGCGACAATTAAGTAAGGCCCAACAAATCGACTTACT
TTCAAATTTATGTAATTTGTTGAAATATGGTTTCACTCTGTATCAAAGTTTTCAATTTTTAAATCTTCAAATGACATATA
AAAATAAGCAATTAGGTACCACCATTCTAAGTGAAATTTCAAATGGTGCACCATGCAATCAAATATTATCACTGATAGGT
TATAGCGATACTATCGTCATGCAAGTATATTTGGCAGAAAGATTTGGCAATATTATAGACGTTCTAGAAGAAACCGTAAA
TTATATGAAAGTGAATAGAAAGTCAGAACAACGATTGTTAAAGAAACTGCAATACCCCTTAATACTAGTTTCTATCTTTA
TTGCTATGATTATTATATTAAACCTCACAGTAATTCCACAGTTTCAACAATTATATACTTCTATGAATATTCAACTATCT
TCTTTTCAAAAAACATTGTCTTTTTTCATTACCAGCTTACCTACTATAATTGTAGTAATGCTCATAATAGTATCTATGTT
GGCTATTATTATGAAATTAATTTATAACAATTTAAATATGCTCAATAAGATAAACTTTGTGATGAAACTACCGCTAATAT
CAGGCTATTTCCAATTATTTAAAACTTATTTTGTAACTAATGAATTAGTGTTGTTTTATAAAAATGGTATTACACTTCAA
TCAATAGTAGACGTTTATATTAACCATAGTAGTGATCCATTTAGACAGTTTCTAGGTAAATACTTATTAACTTATTCAGA
AATGGGATATGGTTTGCCTCAAATTTTAGAAAAACTAAAATGCTTTAAGCCTCAATTAATTAAGTTTGTGCTACAAGGTG
AAAAGAGAGGGAAGCTAGAAGTAGAACTAAAGTTATATTCGCAAATATTAGTAAAACAAATAGAAGATAAAGCGATAAAA
CAGACTCAGTTTTTACAGCCTATTTTATTTTTGATTTTAGGTTTATTTATTGTCGCAATTTATTTAGTAATTATGTTACC
AATGTTTCAAATGATGCAAAGTATAAAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(27-146)

Type II secretion system (T2SS), protein F (T2SSF)

(227-347)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB Staphylococcus aureus MW2

99.438

100

0.994
  comGB Staphylococcus aureus N315

99.438

100

0.994

Multiple sequence alignment