Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   EW029_RS08200 Genome accession   NZ_LR130513
Coordinates   1656213..1656899 (-) Length   228 a.a.
NCBI ID   WP_075583650.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BPH2900 isolate BPH2900     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1651213..1661899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW029_RS08180 rpsT 1652127..1652378 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  EW029_RS08185 holA 1652423..1653397 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  EW029_RS08190 comEC 1653454..1655655 (-) 2202 WP_001670363.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EW029_RS08195 - 1655660..1656121 (-) 462 WP_000439693.1 ComE operon protein 2 -
  EW029_RS08200 comEA 1656213..1656899 (-) 687 WP_075583650.1 ComEA family DNA-binding protein Machinery gene
  EW029_RS08205 - 1656939..1657655 (-) 717 WP_000084824.1 class I SAM-dependent DNA methyltransferase -
  EW029_RS08210 rsfS 1657658..1658011 (-) 354 WP_001088022.1 ribosome silencing factor -
  EW029_RS08215 yqeK 1658012..1658596 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EW029_RS08220 nadD 1658586..1659155 (-) 570 WP_000725167.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  EW029_RS08225 yhbY 1659158..1659448 (-) 291 WP_000955235.1 ribosome assembly RNA-binding protein YhbY -
  EW029_RS08230 aroE 1659452..1660258 (-) 807 WP_000666749.1 shikimate dehydrogenase -
  EW029_RS08235 yqeH 1660272..1661372 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25787.53 Da        Isoelectric Point: 9.6753

>NTDB_id=1116867 EW029_RS08200 WP_075583650.1 1656213..1656899(-) (comEA) [Staphylococcus aureus strain BPH2900 isolate BPH2900]
MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKLEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTI

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1116867 EW029_RS08200 WP_075583650.1 1656213..1656899(-) (comEA) [Staphylococcus aureus strain BPH2900 isolate BPH2900]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAGTGGAAGTTATATATTATAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAATTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAAGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGGAGTAAACAGTGTGCATGTAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACCGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTAGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGATATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

99.123

100

0.991
  comEA Staphylococcus aureus N315

98.246

100

0.982

Multiple sequence alignment