Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EW032_RS07730 Genome accession   NZ_LR130509
Coordinates   1554649..1555146 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain BPH2760 isolate BPH2760     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1549649..1560146
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW032_RS07705 gcvPB 1549811..1551283 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  EW032_RS07710 gcvPA 1551276..1552622 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EW032_RS07715 gcvT 1552642..1553733 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  EW032_RS07720 - 1553892..1554416 (-) 525 WP_001015121.1 shikimate kinase -
  EW032_RS07725 - 1554406..1554552 (-) 147 WP_001789879.1 hypothetical protein -
  EW032_RS07730 comGF 1554649..1555146 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW032_RS07735 comGE 1555064..1555363 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  EW032_RS07740 comGD 1555350..1555796 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  EW032_RS07745 comGC 1555774..1556085 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EW032_RS07750 comGB 1556099..1557169 (-) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW032_RS07755 comGA 1557141..1558115 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EW032_RS07760 - 1558167..1558790 (-) 624 WP_129621228.1 MBL fold metallo-hydrolase -
  EW032_RS07765 - 1558787..1559116 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EW032_RS07770 - 1559116..1560102 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=1116775 EW032_RS07730 WP_001789864.1 1554649..1555146(-) (comGF) [Staphylococcus aureus strain BPH2760 isolate BPH2760]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1116775 EW032_RS07730 WP_001789864.1 1554649..1555146(-) (comGF) [Staphylococcus aureus strain BPH2760 isolate BPH2760]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment