Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   EW024_RS06760 Genome accession   NZ_LR130237
Coordinates   1313146..1314471 (-) Length   441 a.a.
NCBI ID   WP_011528851.1    Uniprot ID   A0A660A486
Organism   Streptococcus pyogenes strain SP444 isolate SP444     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1308146..1319471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW024_RS06750 hpf 1311881..1312429 (-) 549 WP_002988974.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  EW024_RS06755 - 1312509..1313174 (-) 666 WP_011528850.1 ComF family protein -
  EW024_RS06760 comFA/cflA 1313146..1314471 (-) 1326 WP_011528851.1 DEAD/DEAH box helicase Machinery gene
  EW024_RS06765 - 1314527..1315159 (+) 633 WP_042769749.1 YigZ family protein -
  EW024_RS06770 cysK 1315287..1316228 (+) 942 WP_011528853.1 cysteine synthase A -
  EW024_RS06775 - 1316246..1316623 (-) 378 WP_002983674.1 S1 RNA-binding domain-containing protein -
  EW024_RS06780 - 1316623..1318023 (-) 1401 WP_021299066.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  EW024_RS06785 - 1318060..1318701 (-) 642 WP_002983671.1 response regulator transcription factor -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 49854.31 Da        Isoelectric Point: 10.0084

>NTDB_id=1116576 EW024_RS06760 WP_011528851.1 1313146..1314471(-) (comFA/cflA) [Streptococcus pyogenes strain SP444 isolate SP444]
MESIENSYGRLLLESQLPDSARQLAQPLKSVVISRGKMICQRCHYQLDEEARLPSGAYYCRFCLVFGRNQSDKLLYAIPP
MHFPKGNYLVWSGQLTAYQEMISQQLLINMQNQKTTLVHAVTGAGKTEMIYAAIEAVINTGGWVCIASPRVDVCVEVATR
LSQAFSCSICLMHAESFPYQRAPIIVATTHQLLKFHKAFDLLIIDEVDAFPFVNNIQLHYAASQALKEGGAKILLTATST
RTLERKVNKGEVVKLTLARRFHNRPLVIPKFIRSFNLFKMIHRQKLPPKILKYLKKQRKTGYPLLIFLPTILMAESVTAI
LKELLPAEQIACVSSQSQNRKEDITAFRQGKKTILITTTILERGVTFPQIDVFVLGSHHRVYSSQSLVQIAGRVGRSIDR
PDGTLYFFHEGISKAMLLARKEIKEMNYKGYSHDLSTMSTN

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=1116576 EW024_RS06760 WP_011528851.1 1313146..1314471(-) (comFA/cflA) [Streptococcus pyogenes strain SP444 isolate SP444]
ATGGAGTCTATTGAAAATAGTTATGGGCGGTTGTTGCTAGAAAGCCAGTTACCAGATTCAGCAAGGCAGCTTGCTCAACC
TTTAAAAAGTGTAGTAATATCGAGAGGAAAAATGATTTGTCAACGTTGTCACTATCAGCTTGATGAAGAAGCACGATTGC
CAAGTGGAGCCTATTATTGCCGATTTTGTCTTGTCTTTGGACGAAATCAATCAGATAAATTGCTCTATGCTATCCCACCT
ATGCATTTTCCAAAAGGTAATTATCTGGTTTGGAGCGGTCAACTAACTGCTTATCAAGAGATGATTTCCCAACAATTATT
AATCAATATGCAAAATCAAAAAACAACATTAGTTCATGCTGTAACTGGTGCAGGAAAAACAGAAATGATTTATGCTGCGA
TTGAGGCGGTAATTAACACAGGTGGCTGGGTTTGCATTGCCAGCCCAAGAGTAGATGTTTGTGTAGAGGTGGCTACTCGT
TTATCCCAAGCCTTCTCCTGCAGCATCTGTTTAATGCACGCAGAGTCTTTTCCTTATCAAAGAGCTCCTATTATAGTAGC
CACAACGCATCAGCTGCTAAAATTTCACAAGGCTTTTGATCTCTTAATTATTGATGAAGTAGACGCATTCCCTTTTGTAA
ATAATATTCAACTTCATTATGCTGCAAGTCAGGCTTTAAAAGAAGGTGGCGCAAAGATTTTATTAACTGCAACTTCAACT
AGAACTTTAGAAAGAAAGGTAAACAAGGGAGAAGTAGTCAAGCTGACATTGGCGAGACGCTTTCATAATAGGCCATTAGT
TATTCCGAAATTTATAAGAAGTTTTAATCTATTTAAAATGATACATCGGCAAAAACTTCCTCCTAAAATTTTAAAATACT
TAAAAAAACAAAGAAAAACAGGCTATCCCCTTTTAATTTTTCTACCCACTATTCTAATGGCAGAATCTGTTACAGCAATC
TTAAAAGAGTTATTGCCCGCCGAACAAATAGCTTGTGTTTCTAGTCAATCACAAAACAGGAAAGAAGATATCACAGCTTT
TCGTCAAGGGAAAAAAACGATTTTAATTACAACAACAATTTTAGAAAGGGGTGTGACATTTCCCCAAATTGATGTTTTTG
TTCTTGGGTCTCATCATCGTGTCTATAGTTCCCAAAGTCTTGTTCAAATTGCTGGACGAGTTGGAAGATCTATAGACAGA
CCAGACGGAACGTTATATTTCTTTCATGAAGGTATCAGTAAAGCAATGCTACTAGCAAGAAAAGAGATTAAAGAAATGAA
TTATAAAGGGTATTCGCATGATCTGTCTACTATGTCAACAAATTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(289-396)

DEAD/DEAH box helicase (DEAD)

(98-244)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A660A486

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae D39

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae R6

54.415

95.011

0.517
  comFA/cflA Streptococcus pneumoniae TIGR4

54.415

95.011

0.517
  comFA/cflA Streptococcus mitis NCTC 12261

53.081

95.692

0.508
  comFA/cflA Streptococcus mitis SK321

52.133

95.692

0.499
  comFA Lactococcus lactis subsp. cremoris KW2

47.368

90.476

0.429
  comFA Latilactobacillus sakei subsp. sakei 23K

39.851

91.61

0.365

Multiple sequence alignment