Detailed information NTG1116552

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: EW024_RS01425
• Genome accession: NZ_LR130237
• Coordinates: 261569..262639 (+)
• Length: 356 a.a.
• NCBI ID: WP_010921893.1
• Uniprot ID: A0A4U9C068
• Organism: Streptococcus pyogenes strain SP444 isolate SP444
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 256569..267639

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EW024_RS01410	amiA	257088..259067 (+)	1980	WP_021299351.1	peptide ABC transporter substrate-binding protein	Regulator
EW024_RS01415	amiC	259132..260634 (+)	1503	WP_011017334.1	ABC transporter permease	Regulator
EW024_RS01420	amiD	260634..261560 (+)	927	WP_002986004.1	oligopeptide ABC transporter permease OppC	Regulator
EW024_RS01425	amiE	261569..262639 (+)	1071	WP_010921893.1	ABC transporter ATP-binding protein	Regulator
EW024_RS01430	amiF	262632..263555 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
EW024_RS09565	-	263593..263676 (-)	84	Protein_229	IS3 family transposase	-
EW024_RS09570	-	263810..264129 (-)	320	Protein_230	IS3 family transposase	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39703.77 Da
• Isoelectric Point: 5.6707

>NTDB_id=1116552 EW024_RS01425 WP_010921893.1 261569..262639(+) (amiE) [Streptococcus pyogenes strain SP444 isolate SP444]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=1116552 EW024_RS01425 WP_010921893.1 261569..262639(+) (amiE) [Streptococcus pyogenes strain SP444 isolate SP444]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATTGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTCATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGGAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A4U9C068

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537