Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   E0E14_RS08360 Genome accession   NZ_LR027877
Coordinates   1664057..1664743 (-) Length   228 a.a.
NCBI ID   WP_001792568.1    Uniprot ID   A0A133PXY3
Organism   Staphylococcus aureus isolate BPH3244     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1659057..1669743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E0E14_RS08340 rpsT 1659971..1660222 (+) 252 WP_001274017.1 30S ribosomal protein S20 -
  E0E14_RS08345 holA 1660267..1661241 (-) 975 WP_001282562.1 DNA polymerase III subunit delta -
  E0E14_RS08350 comEC 1661298..1663499 (-) 2202 WP_001566051.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  E0E14_RS08355 - 1663504..1663965 (-) 462 WP_000439693.1 ComE operon protein 2 -
  E0E14_RS08360 comEA 1664057..1664743 (-) 687 WP_001792568.1 ComEA family DNA-binding protein Machinery gene
  E0E14_RS08365 - 1664783..1665499 (-) 717 WP_000084829.1 class I SAM-dependent DNA methyltransferase -
  E0E14_RS08370 rsfS 1665502..1665855 (-) 354 WP_001088022.1 ribosome silencing factor -
  E0E14_RS08375 yqeK 1665856..1666440 (-) 585 WP_001019324.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  E0E14_RS08380 nadD 1666430..1666999 (-) 570 WP_000725162.1 nicotinate (nicotinamide) nucleotide adenylyltransferase -
  E0E14_RS08385 yhbY 1667002..1667292 (-) 291 WP_000955231.1 ribosome assembly RNA-binding protein YhbY -
  E0E14_RS08390 aroE 1667296..1668102 (-) 807 WP_000666761.1 shikimate dehydrogenase -
  E0E14_RS08395 yqeH 1668116..1669216 (-) 1101 WP_001280141.1 ribosome biogenesis GTPase YqeH -

Sequence

Protein


Download         Length: 228 a.a.        Molecular weight: 25863.49 Da        Isoelectric Point: 9.3305

>NTDB_id=1116008 E0E14_RS08360 WP_001792568.1 1664057..1664743(-) (comEA) [Staphylococcus aureus isolate BPH3244]
MVLLYQFLLRYKDFLTQWKLYILSAVVLIMVLIGFIFWRQDDYTSRNFENKDTALKQSTSENSSLSKVEDVQVKDGDNSK
NKGPVYVDVKGAVKHPNVYKMTSKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIEVNSVHEKN
GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKTFDKLKSYFTT

Nucleotide


Download         Length: 687 bp        

>NTDB_id=1116008 E0E14_RS08360 WP_001792568.1 1664057..1664743(-) (comEA) [Staphylococcus aureus isolate BPH3244]
GTGGTTTTATTGTATCAATTTTTATTACGCTATAAAGATTTTTTAACTCAATGGAAGTTATATATTCTAAGTGCTGTTGT
TTTAATTATGGTATTAATTGGTTTTATATTCTGGAGACAAGATGATTATACTTCAAGAAATTTTGAAAATAAAGATACTG
CTCTGAAACAAAGCACTAGTGAAAATAGTAGTTTGTCCAAAGTAGAAGATGTCCAGGTCAAAGATGGAGATAATTCCAAA
AATAAGGGTCCTGTATATGTCGATGTAAAAGGTGCTGTTAAACATCCTAATGTTTATAAAATGACATCTAAGGATAGAGT
AGTTGATTTACTTGATAAAGCACAATTATTGGAGGATGCAGATGTAAGTCAAATTAATTTGTCTGAAAAATTAACAGATC
AAAAAATGATTTTCATACCTCATAAAGGACAAAAGAATGTTGAACCACAAATTGAAGTAAACAGTGTGCACGAAAAAAAT
GGGAACACAAATAATACTAAAGTAAATTTAAATACGGCATCTGTATCAGAATTGATGTCTGTTCCTGGAGTTGGGCAAGC
TAAAGCTAATGCAATTGTTGAATATCGCAACCAACAAGGTGCATTTCAAGAAATTGACGATTTGAAAAAAGTAAAAGGTT
TTGGAAGTAAAACTTTTGATAAACTGAAATCTTATTTCACGACATAA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(164-227)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A133PXY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Staphylococcus aureus MW2

98.238

99.561

0.978
  comEA Staphylococcus aureus N315

97.357

99.561

0.969

Multiple sequence alignment